RNAiDB Tree View

Gene: C02F5.1

C02F5.1	SMap	S_parent	Sequence	C02F5
	Chromosome	III
	Identity (5)
	Gene_info	Gene_class	knl
		Structured_description	Provisional_description	knl-1 encodes a novel acidic protein with a coiled-coil region at its C-terminus; KNL-1 is an essential kinetochore component that is required for proper spindle elongation and chromosome separation, and in the kinetochore assembly pathway, plays a key role in linking the initiation of kinetochore formation with the construction of a functional microtubule-binding interface; in the assembly pathway, KNL-1 functions downstream of the DNA-proximal kinetochore components CeCENP-A/HCP-3 and CeCENP-B/HCP-4 and upstream of the outer kinetochore components HIM-10/Nuf2p, NDC-80/HEC1, CeBUB-1, HCP-1, and CeCLASP2/CLS-2; in expression studies in the one-cell embryo, KNL-1 localizes to kinetochores throughout mitosis.	Paper_evidence	WBPaper00004403
						WBPaper00006142
					Person_evidence	WBPerson1843
					Curator_confirmed	WBPerson1843
					Date_last_updated	17 Jun 2004 00:00:00
			Concise_description	knl-1 encodes a novel acidic protein with a coiled-coil region at its C-terminus; KNL-1 is an essential kinetochore component that is required for proper spindle elongation and chromosome separation, and in the kinetochore assembly pathway, plays a key role in linking the initiation of kinetochore formation with the construction of a functional microtubule-binding interface; in the assembly pathway, KNL-1 functions downstream of the DNA-proximal kinetochore components CeCENP-A/HCP-3 and CeCENP-B/HCP-4 and upstream of the outer kinetochore components HIM-10/Nuf2p, NDC-80/HEC1, CeBUB-1, HCP-1, and CeCLASP2/CLS-2; in expression studies in the one-cell embryo, KNL-1 localizes to kinetochores throughout mitosis.
	Molecular_info	Corresponding_CDS	C02F5.1
		Corresponding_protein	WP:CE02450
		Corresponding_PCR_product	smd_C02F5.1
			sjj_C02F5.1
		Corresponding_RNAi_reagent (7)
	Experimental_info	RNAi_result (5)
		WB_RNAi_result (7)
		Expr_pattern (2)
		Y2H_bait	Y2H010986
			Y2H010987
			Y2H010988
			Y2H010991
	Reference (5)
	Remark	Sequence connection from [Oegema K, Hyman AA], 02/06/010 krb
	Method	Gene