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RNAi: Cenix:221-e5   

Cenix:221-e5RNAi_reagentCenix:221-e5
MethodRNAi
WormBaseIDWBRNAi00038683
ReferenceWBPaper00025054
Experiment LaboratoryTH
Author Sonnichsen B
Koski LB
Walsh A
Marschall P
Neumann B
Brehm M
Alleaume AM
Artelt J
Bettencourt P
Cassin E
Hewitson M
Holz C
Khan M
Lazik S
Martin C
Nitzsche B
Ruer M
Stamford J
Winzi M
Heinkel R
Roder M
Finell J
Hantsch H
Jones SJ
Jones M
Piano F
Gunsalus KC
Oegema K
Gonczy P
Coulson A
Hyman AA
Echeverri CJ
Date24 Mar 2005 00:00:00
Inhibits Predicted_gene (3)
Gene B0035.8 Source_evidenceePCR118WS150 Sequence/Annotation Release
Map_statusBest_ePCRIV1132484011324957
F54E12.4 Source_evidenceePCR118WS150 Sequence/Annotation Release
Map_statusBest_ePCRIV1133983611339953
H02I12.6 Source_evidenceePCR118WS150 Sequence/Annotation Release
Map_statusBest_ePCRIV1140051611400633
Transcript B0035.8 Source_evidenceePCR118WS150 Sequence/Annotation Release
F54E12.4 Source_evidenceePCR118WS150 Sequence/Annotation Release
H02I12.6 Source_evidenceePCR118WS150 Sequence/Annotation Release
WB_Predicted_gene B0035.8 Inferred_automaticallyRNAi_primary
F54E12.4 Inferred_automaticallyRNAi_primary
F55G1.3 Inferred_automaticallyRNAi_primary
H02I12.6 Inferred_automaticallyRNAi_primary
WB_Gene (4)
Gene_target B0035.8 RNAiDBePCRePCR_best
WormbaseBLASTBLAST_wormbase_primary
F54E12.4 RNAiDBePCRePCR_best
WormbaseBLASTBLAST_wormbase_primary
H02I12.6 RNAiDBePCRePCR_best
WormbaseBLASTBLAST_wormbase_primary
F55G1.3 WormbaseBLASTBLAST_wormbase_primary
WB_Homol Homol_homol (4)
Supporting_data External_Movie (8)
Remark early embryonic: Sister Chromatid Separation (Cross-eyed)
early embryonic: Highly reproducible chromatin segregation defect resulting in the typical cross-eyed nuclear separation pattern with tear shaped nuclei that stay attached to the newly forming cortex. Interfering chromatin bridges can result in failure of cytokinesis completion. Phenotype is more pronounced at four cell stage.
90-100% of the progeny die during embryonic development.
DeliveryDelivered_byInjection
Results Phenotype Emb
Sister Chromatid Separation abnormal (Cross-eyed)
Phenotype_summary (7)
DB_infoDatabasePhenobank2 Gene&RNAID GeneID=502287&RNAID=3505983
GeneID=509520&RNAID=3505983
GeneID=510211&RNAID=3505983