RNAiDB Tree View

Gene: F54F7.5

F54F7.5	SMap	S_parent	Sequence	F54F7
	Chromosome	X
	Identity (5)
	Gene_info	Gene_class	mes
		Allele (7)
		Phenotype	[C.elegansII] bn7ts : grandchildless, at 25C, 70% of progeny develop into sterile adults;P2 and P3 divisions variably defective, P4 transformed into D-like muscle precursor,generating additional muscle cells, no germ cells. OA9: bn74 (deletion null, ts impenetrant Mes resembling bn7), bn24, q222,etc. (all ts, impenetrant). Cloned: cosmid rescue C38D5. [Strome et al. 1995]
		Strain	SS149
		GO_term (11)
		Structured_description	Provisional_description	The mes-1 gene encodes a receptor tyrosine kinase-like protein that is required for unequal cell divisions in the early embryonic germline; during embryonic cell divisions, mes-1 is involved in positioning of the early mitotic spindle and of associated P granules.	Paper_evidence	WBPaper00004323
					Person_evidence	WBPerson567
					Curator_confirmed	WBPerson567
					Date_last_updated	17 Jun 2004 00:00:00
				mes-1 mutant embryos show transformation of the germline blastomere P4 into a muscle precursor, like its somatic sister.
				The defect in P4 is preceded by lost polarity in cell divisions of the earlier germline blastomeres P2 and P3; in mes-1 embryos, P2 and P3 (abnormally) undergo symmetric divisions and partition germ granules to both daughters.
				Consistent with MES-1 being kinase-like, it contains a signal sequence, a single transmembrane domain, and an intracellular kinase-like domain.
				The extracellular region of MES-1 contains seven potential glycosylation sites (consensus NXS/T where X is any amino acid except P and D)but no recognizable domains or motifs.
				However, MES-1 lacks several amino acids required for catalytic activity within the kinase subdomains, which suggests that MES-1 is not a functional kinase.
				In wild-type four- to 24-cell embryos, MES-1 protein resides in a crescent at the junction between the germline cell and its neighboring gut cell; this localization does not genetically require a correctly specified fate in either the germline or the gut blastomere, but is genetically dependent on correct correct spatial organization of the embryo.
				MES-1 has thus been proposed to directly orient the mitotic spindle and its associated P granules in early embryonic germline blastomeres.
			Concise_description	The mes-1 gene encodes a receptor tyrosine kinase-like protein that is required for unequal cell divisions in the early embryonic germline; during embryonic cell divisions, mes-1 is involved in positioning of the early mitotic spindle and of associated P granules.
	Molecular_info (6)
	Experimental_info (5)
	Reference (43)
	Remark	Sequence connection from [Berkowitz LA, Strome S]
	Method	Gene