Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 

Gene: F59B2.6   

F59B2.6 Evidence Paper_evidenceWBPaper00006004
CGC_data_submission
SMapS_parentSequenceF59B2
ChromosomeIII
Identity (5)
Gene_info Gene_classzif
Allele gk117 Inferred_automaticallyFrom strain object: VC141
tm2018
uCE3-1089
StrainVC141
Structured_description Provisional_description zif-1 encodes a SOCS-box protein that promotes establishment of the germ line by targetting germline proteins for cullin-dependent degradation in non-germline (somatic) cells; ZIF-1 binds germline CCCH-(zinc)-finger proteins and the E3 ubiquitin ligase subunit elongin C (ELC-1); while ZIF-1 has no obvious homologs, it may be analogous to the Drosophila SOCS-box protein GUSTAVUS, which is required for VASA localization to the germline and which does have mammalian homologs. Paper_evidence WBPaper00006004
WBPaper00013191
Person_evidenceWBPerson567
Curator_confirmedWBPerson567
Date_last_updated17 Jun 2004 00:00:00
In yeast two-hybrid assays, ZIF-1 interacted with the zinc finger domains of PIE-1, POS-1, MEX-1 and MEX-5, but not TIS-11, by a mechanism that appears to recognize specific CCCH fingers in the degraded proteins.
zif-1(RNAi) embryos abnormally maintain PIE-1, POS-1, MEX-1, MEX-5, MEX-6, and GFP::PIE-1(ZF1) and GFP::MEX-5(ZF2) fusions in somatic blastomeres; however, zif-1(RNAi) embryos show no effect on germline proteins that lack CCCH fingers (PAR-2 and PGL-1), on asymmetric distribution of CCCH finger proteins to germline blastomeres, or on the degradation of OMA-1 (a CCCH finger protein) in all cells after the one-cell stage.
zif-1(RNAi) animals die during embryogenesis.
Concise_descriptionzif-1 encodes a SOCS-box protein that promotes establishment of the germ line by targetting germline proteins for cullin-dependent degradation in non-germline (somatic) cells; ZIF-1 binds germline CCCH-(zinc)-finger proteins and the E3 ubiquitin ligase subunit elongin C (ELC-1); while ZIF-1 has no obvious homologs, it may be analogous to the Drosophila SOCS-box protein GUSTAVUS, which is required for VASA localization to the germline and which does have mammalian homologs.
Molecular_info (5)
Experimental_info RNAi_result Cenix:308-b1
JA:F59B2.6
TH:308B1
WB_RNAi_result (5)
Reference (7)
Remark Gene name created from parsing 'genotype' field from CGC strain information [krb 020721]
Gene name created from parsing 'genotype' field from CGC strain informationCGC_data_submission
Map position created from combination of previous interpolated map position (based on known location of sequence) and allele information. Therefore this is not a genetic map position based on recombination frequencies or genetic experiments. This was done on advice of the CGC.CGC_data_submission
MethodGene