H06O01.1 | SMap | S_parent | Sequence | SUPERLINK_CB_I
|
| Chromosome | I |
|
Identity (5)
|
|
Gene_info
| Gene_class | pdi
|
| |
GO_term
| GO:0016853
| IEA
| Inferred_automatically |
| | | GO:0005783
| IEA
| Inferred_automatically |
| | | GO:0005489
| IEA
| Inferred_automatically |
| | | GO:0006118
| IEA
| Inferred_automatically |
| |
Structured_description
|
Provisional_description
| pdi-3 encodes a protein disulfide isomerase (PDI) required for normal cuticle collagen deposition and, subliminally, for maintenance of normal body shape; PDI-3 has both PDI and calcium-dependent transglutaminase activity in vitro, crosslinking proteins through a gamma-glutamyl epsilon-lysine dual residue; PDI-3 is homologous to the human PDI ERp60, as well as a homolog in the dog heartworm Dirofilaria immitis that is required for larval molting; the joint PDI/transglutaminase activity of PDI-3 is biochemically conserved in nematodes and mammals; pdi-3 is constitutively and broadly expressed, particularly in gut and hypodermis. |
Paper_evidence (8)
|
| | | | | Person_evidence | WBPerson567
|
| | | | | Curator_confirmed | WBPerson567
|
| | | | | Date_last_updated | 17 Jun 2004 00:00:00 |
| | | | While pdi-3(RNAi) animals have no grossly obvious phenotype in mass RNAi assays, their localization of the collagen DPY-7 is mildly abnormal in annular furrows of the cuticle. |
| | | | More strikingly, pdi-3(RNAi) animals have strong, specific phenotypes with RNAi in a mutant background of alleles with weakened cuticles, such as sqt-3(e2117), dpy-18(e364), dpy-18(ok162), or dpy-18(bx26). |
| | | | pdi-3(RNAi) animals with a dpy-18(-) or sqt-3(-) background are severely dumpy, with disorderly lateral alae, abnormally weak cuticle, erratically distributed lateral seam cells, protruding vulvas, egg-laying defects, and abnormally slow growth. |
| | | | Defective tails are seen with sqt-3(-). |
| | | | These defects are not observed for background mutations of prolyl isomerase subunits other than DPY-18, or for mutations of collagens other than sqt-3. |
| | | | The second cysteine residue of the Cys-Gly-His-Cys motif in the thioredoxin domain of PDI-3 is the active site of the transamidation reaction; chemical modification of the His residue in this motif blocks transglutaminase activity. |
| | | | The PDI-3 protein is predicted to be mitochondrial with 52% accuracy. |
| | | | Proteins localized to a given organelle by experiments tend to share a characteristic phylogenetic distribution of their homologs, called a phylogenetic profile. |
| | | | For instance, mitochondrial proteins can be identified effectively by their phylogenetic profiles. |
| | | | By applying phylogenetic profile analysis to the C. elegans genome, Eisenberg and coworkers inferred that this gene, along with 2-4% of all the nuclear genes in C. elegans, encodes a mitochondrial protein. |
| | | | The large fraction of organism-specific and eukaryote-derived genes suggests that C. elegans mitochondria perform specialized roles absent from prokaryotic mitochondrial ancestors. |
| | | Concise_description | pdi-3 encodes a protein disulfide isomerase (PDI) required for normal cuticle collagen deposition and, subliminally, for maintenance of normal body shape; PDI-3 has both PDI and calcium-dependent transglutaminase activity in vitro, crosslinking proteins through a gamma-glutamyl epsilon-lysine dual residue; PDI-3 is homologous to the human PDI ERp60, as well as a homolog in the dog heartworm Dirofilaria immitis that is required for larval molting; the joint PDI/transglutaminase activity of PDI-3 is biochemically conserved in nematodes and mammals; pdi-3 is constitutively and broadly expressed, particularly in gut and hypodermis. |
|
Molecular_info (5)
|
|
Experimental_info
|
RNAi_result (5)
|
| |
WB_RNAi_result (6)
|
| |
Expr_pattern (5)
|
| |
Y2H_target (3)
|
|
Reference
| WBPaper00005704
|
| | WBPaper00024269
|
| | WBPaper00006361
|
| | WBPaper00026640
|
| Method | Gene
|