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Gene: M7.1   

M7.1SMapS_parentSequenceM7
ChromosomeIV
Identity (5)
Gene_info Gene_classlet
Reference_alleles1132
Allele s689
s1132 Inferred_automaticallyFrom strain object: BC2020
s2293
Phenotype (5)
StrainBC2020
GO_term GO:0008642 IEA Inferred_automatically
GO:0006464 IEA Inferred_automatically
GO:0006512 IEA Inferred_automatically
Contained_in_operonCEOP4404
Structured_description Provisional_description let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system. Paper_evidence (5)
Curator_confirmedWBPerson1843
Date_last_updated09 May 2005 00:00:00
let-70 expression in Saccharomyces cerevisiae strains doubly mutant for ubc4 and ubc5 can rescue growth defects seen in this mutant strain at 30 and 37 degrees C.
Concise_descriptionlet-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system.
Molecular_info (6)
Experimental_info RNAi_result (7)
WB_RNAi_result (15)
Expr_pattern Expr725
Expr726
Expr1511
Y2H_target Y2H008659
Y2H008660
Y2H008883
Reference (18)
Remark [BC Lab] Essential gene. Phenotype of different homozygous alleles range from early to mid larval development.
This gene is now the primary name again for ubc-2. Previously, let-70 was made an 'Other_name' for ubc-2. 02/06/12 krb.
MethodGene