M7.1 | SMap | S_parent | Sequence | M7
|
| Chromosome | IV |
|
Identity (5)
|
|
Gene_info
| Gene_class | let
|
| | Reference_allele | s1132
|
| |
Allele
| s689
|
| | | s1132
| Inferred_automatically | From strain object: BC2020 |
| | | s2293
|
| |
Phenotype (5)
|
| | Strain | BC2020
|
| |
GO_term
| GO:0008642
| IEA
| Inferred_automatically |
| | | GO:0006464
| IEA
| Inferred_automatically |
| | | GO:0006512
| IEA
| Inferred_automatically |
| | Contained_in_operon | CEOP4404
|
| |
Structured_description
|
Provisional_description
| let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system. |
Paper_evidence (5)
|
| | | | | Curator_confirmed | WBPerson1843
|
| | | | | Date_last_updated | 09 May 2005 00:00:00 |
| | | | let-70 expression in Saccharomyces cerevisiae strains doubly mutant for ubc4 and ubc5 can rescue growth defects seen in this mutant strain at 30 and 37 degrees C. |
| | | Concise_description | let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system. |
|
Molecular_info (6)
|
|
Experimental_info
|
RNAi_result
| Cenix:186-e7
|
| | | JA:M7.1
|
| | | Simmer:M7.1
|
| | | WBRNAi00060823
|
| | | WBRNAi00060824
|
| | | WBRNAi00060825
|
| | | WBRNAi00060826
|
| |
WB_RNAi_result (15)
|
| |
Expr_pattern
| Expr725
|
| | | Expr726
|
| | | Expr1511
|
| |
Y2H_target
| Y2H008659
|
| | | Y2H008660
|
| | | Y2H008883
|
|
Reference (18)
|
|
Remark
| [BC Lab] Essential gene. Phenotype of different homozygous alleles range from early to mid larval development. |
| | This gene is now the primary name again for ubc-2. Previously, let-70 was made an 'Other_name' for ubc-2. 02/06/12 krb. |
| Method | Gene
|