RNAi: PF:GL1_1A12
PF:GL1_1A12 | RNAi_reagent | sjj_F22B3.4 | ||||||
---|---|---|---|---|---|---|---|---|
Method | RNAi | |||||||
WormBaseID | WBRNAi00007543 | |||||||
Gene_set | GL1 | |||||||
Reference | WBPaper00005599 | |||||||
Experiment | Laboratory | KK | ||||||
Date | 19 Nov 2002 00:00:00 | |||||||
Inhibits | Predicted_gene | F22B3.4 | Source_evidence | ePCR | 798 | WS150 Sequence/Annotation Release | ||
Gene | F22B3.4 | Source_evidence | ePCR | 827 | WS150 Sequence/Annotation Release | |||
Map_status | Unique_ePCR | IV | 11411918 | 11412845 | ||||
Transcript | F22B3.4 | Source_evidence | ePCR | 827 | WS150 Sequence/Annotation Release | |||
WB_Predicted_gene | F07A11.2a | Inferred_automatically | RNAi_secondary | |||||
F07A11.2b | Inferred_automatically | RNAi_secondary | ||||||
F22B3.4 | Inferred_automatically | RNAi_primary | ||||||
WB_Gene | F07A11.2 | Inferred_automatically | RNAi_secondary | |||||
F22B3.4 | Inferred_automatically | RNAi_primary | ||||||
Gene_target | F22B3.4 | RNAiDB | ePCR | ePCR_unique | ||||
Wormbase | BLAST | BLAST_wormbase_primary | ||||||
F07A11.2a | Wormbase | BLAST | BLAST_wormbase_secondary | |||||
F07A11.2b | Wormbase | BLAST | BLAST_wormbase_secondary | |||||
F07A11.2 | Wormbase | BLAST | BLAST_wormbase_secondary | |||||
WB_Homol | Homol_homol | Y73B3A:RNAi | ||||||
F07A11:RNAi | ||||||||
F22B3:RNAi | ||||||||
Supporting_data | Movie | 1a12-01.mov | ||||||
1a12-03.mov | ||||||||
1a12rep-0X.mov | ||||||||
Remark | PCR product contains flanking T7 promoter sequences | |||||||
Delivery | Delivered_by | Injection | ||||||
Results | Phenotype | Abnormal one-cell shape | ||||||
Catastrophic one-cell arrest | ||||||||
Cytokinesis defect | ||||||||
Emb | Remark | % penetrance range | ||||||
Penetrance | 80 | 100 | ||||||
Multiple nuclei in 1, 2, or 4-cell embryos | ||||||||
Osmotically/ pressure sensitive | ||||||||
Polar body reabsorbed | ||||||||
Ste | ||||||||
No developing embryos seen 48 hours after dsRNA injection | ||||||||
Reduced fecundity of injected worm | ||||||||
Phenotypic_signature | Discrete_pheno_char (47) | |||||||
Phenotype_summary (7) |