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RNAi: PF:GL1_3A1   

PF:GL1_3A1RNAi_reagentsjj_F49E11.1
MethodRNAi
WormBaseIDWBRNAi00007724
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits (6)
WB_HomolHomol_homolF49E11:RNAi
WB_Uniquely_mapped
Supporting_data Movie 3a1-03.mov
3a1-0101.mov
3a1-0201.mov
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Cytokinesis defect
Dpy RemarkObserved in <10% of progeny.
Ectopic cleavage furrows
Emb Remark% penetrance range
Penetrance80100
EMS fails to extend anteriorly
Exaggerated pseudocleavage
Excessive blebbing
Loose mitotic furrow
Multiple nuclei in 1, 2, or 4-cell embryos
P0 spindle does not rotate
Pronuclear breakdown asynchronous
Rup RemarkObserved in <10% of progeny.
Stp RemarkObserved in >=10% of progeny.
Phenotypic_signature Discrete_pheno_char 1No
2No
3No
4No
5No
6No
7Yes
8Yes
9No
10No
11No
12No
13Yes
14No
15No
16No
17No
18No
19No
20No
21No
22No
23Yes
24No
25No
26No
27No
28Yes
29No
30Yes
31Yes
32Yes
33No
34No
35No
36No
37Yes
38No
39No
40No
41Yes
42No
43No
44No
45No
46Yes
47No
Phenotype_summary (7)