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RNAi: PF:GL1_3A1   

PF:GL1_3A1RNAi_reagentsjj_F49E11.1
MethodRNAi
WormBaseIDWBRNAi00007724
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits (6)
WB_HomolHomol_homolF49E11:RNAi
WB_Uniquely_mapped
Supporting_data Movie 3a1-03.mov
3a1-0101.mov
3a1-0201.mov
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Cytokinesis defect
Dpy RemarkObserved in <10% of progeny.
Ectopic cleavage furrows
Emb Remark% penetrance range
Penetrance80100
EMS fails to extend anteriorly
Exaggerated pseudocleavage
Excessive blebbing
Loose mitotic furrow
Multiple nuclei in 1, 2, or 4-cell embryos
P0 spindle does not rotate
Pronuclear breakdown asynchronous
Rup RemarkObserved in <10% of progeny.
Stp RemarkObserved in >=10% of progeny.
Phenotypic_signature Discrete_pheno_char (47)
Phenotype_summary Ste (2)
StpResultYes
EmbResultYes
EmbPhenoResultYes
Emb_image_dataResultYes
PostembPhenoResultYes
Movies_imagesResultYes