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RNAi: PF:GL1_4B11 
| PF:GL1_4B11 | RNAi_reagent | sjj_F56A3.4 | ||||||
|---|---|---|---|---|---|---|---|---|
| Method | RNAi | |||||||
| WormBaseID | WBRNAi00007829 | |||||||
| Gene_set | GL1 | |||||||
| Reference | WBPaper00005599 | |||||||
| Experiment | Laboratory | KK | ||||||
| Date | 19 Nov 2002 00:00:00 | |||||||
| Inhibits | Predicted_gene | F56A3.4 | Source_evidence | ePCR | 1121 | WS150 Sequence/Annotation Release | ||
| Gene | F56A3.4 | Source_evidence | ePCR | 1121 | WS150 Sequence/Annotation Release | |||
| Map_status | Unique_ePCR | I | 5174153 | 5175320 | ||||
| Transcript | F56A3.4 | Source_evidence | ePCR | 1121 | WS150 Sequence/Annotation Release | |||
| WB_Predicted_gene | F56A3.4 | Inferred_automatically | RNAi_primary | |||||
| WB_Gene | F56A3.4 | Inferred_automatically | RNAi_primary | |||||
| Gene_target | F56A3.4 | RNAiDB | ePCR | ePCR_unique | ||||
| Wormbase | BLAST | BLAST_wormbase_primary | ||||||
| WB_Homol | Homol_homol | F56A3:RNAi | ||||||
| WB_Uniquely_mapped | ||||||||
| Supporting_data | Movie | 4b11-09.mov | ||||||
| 4b11-0701.mov | ||||||||
| 4b11-0801.mov | ||||||||
| Remark | PCR product contains flanking T7 promoter sequences | |||||||
| Delivery | Delivered_by | Injection | ||||||
| Results | Phenotype | Cytokinesis defect | ||||||
| Ectopic cleavage furrows | ||||||||
| Emb | Remark | % penetrance range | ||||||
| Penetrance | 80 | 100 | ||||||
| Excessive blebbing | ||||||||
| Gro | Remark | Observed in >=10% of progeny. | ||||||
| Large cytoplasmic granules | ||||||||
| Loose mitotic furrow | ||||||||
| Multiple nuclei in 1, 2, or 4-cell embryos | ||||||||
| P0 spindle absent | ||||||||
| P0 spindle positioning defect | ||||||||
| Polar body reabsorbed | ||||||||
| Pronuclei fail to migrate | ||||||||
| Phenotypic_signature | Discrete_pheno_char (47) | |||||||
| Phenotype_summary (7) |
