RNAi: PF:GL1_4B11
PF:GL1_4B11 | RNAi_reagent | sjj_F56A3.4 | ||||||
---|---|---|---|---|---|---|---|---|
Method | RNAi | |||||||
WormBaseID | WBRNAi00007829 | |||||||
Gene_set | GL1 | |||||||
Reference | WBPaper00005599 | |||||||
Experiment (2) | ||||||||
Inhibits | Predicted_gene | F56A3.4 | Source_evidence | ePCR | 1121 | WS150 Sequence/Annotation Release | ||
Gene | F56A3.4 | Source_evidence | ePCR | 1121 | WS150 Sequence/Annotation Release | |||
Map_status | Unique_ePCR | I | 5174153 | 5175320 | ||||
Transcript | F56A3.4 | Source_evidence | ePCR | 1121 | WS150 Sequence/Annotation Release | |||
WB_Predicted_gene | F56A3.4 | Inferred_automatically | RNAi_primary | |||||
WB_Gene | F56A3.4 | Inferred_automatically | RNAi_primary | |||||
Gene_target | F56A3.4 | RNAiDB | ePCR | ePCR_unique | ||||
Wormbase | BLAST | BLAST_wormbase_primary | ||||||
WB_Homol | Homol_homol | F56A3:RNAi | ||||||
WB_Uniquely_mapped | ||||||||
Supporting_data | Movie (3) | |||||||
Remark | PCR product contains flanking T7 promoter sequences | |||||||
Delivery | Delivered_by | Injection | ||||||
Results | Phenotype | Cytokinesis defect | ||||||
Ectopic cleavage furrows | ||||||||
Emb | Remark | % penetrance range | ||||||
Penetrance | 80 | 100 | ||||||
Excessive blebbing | ||||||||
Gro | Remark | Observed in >=10% of progeny. | ||||||
Large cytoplasmic granules | ||||||||
Loose mitotic furrow | ||||||||
Multiple nuclei in 1, 2, or 4-cell embryos | ||||||||
P0 spindle absent | ||||||||
P0 spindle positioning defect | ||||||||
Polar body reabsorbed | ||||||||
Pronuclei fail to migrate | ||||||||
Phenotypic_signature | Discrete_pheno_char | 1 | No | |||||
2 | No | |||||||
3 | No | |||||||
4 | No | |||||||
5 | No | |||||||
6 | Yes | |||||||
7 | Yes | |||||||
8 | Yes | |||||||
9 | No | |||||||
10 | No | |||||||
11 | No | |||||||
12 | Unsure | |||||||
13 | Unsure | |||||||
14 | No | |||||||
15 | Unsure | |||||||
16 | Yes | |||||||
17 | Unsure | |||||||
18 | Unsure | |||||||
19 | Unsure | |||||||
20 | Unsure | |||||||
21 | No | |||||||
22 | No | |||||||
23 | Unsure | |||||||
24 | Yes | |||||||
25 | No | |||||||
26 | Yes | |||||||
27 | Yes | |||||||
28 | Not applicable | |||||||
29 | Not applicable | |||||||
30 | Yes | |||||||
31 | Yes | |||||||
32 | Yes | |||||||
33 | No | |||||||
34 | Not applicable | |||||||
35 | Not applicable | |||||||
36 | Not applicable | |||||||
37 | Not applicable | |||||||
38 | Not applicable | |||||||
39 | Not applicable | |||||||
40 | Not applicable | |||||||
41 | Not applicable | |||||||
42 | Not applicable | |||||||
43 | Not applicable | |||||||
44 | Not applicable | |||||||
45 | Not applicable | |||||||
46 | Not applicable | |||||||
47 | No | |||||||
Phenotype_summary | Ste | Result | No | |||||
Reason | None_detected | |||||||
Stp | Result | No | ||||||
Reason | None_detected | |||||||
Emb | Result | Yes | ||||||
EmbPheno | Result | Yes | ||||||
Emb_image_data | Result | Yes | ||||||
PostembPheno | Result | Yes | ||||||
Movies_images | Result | Yes |