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RNAi: PF:GL1_4B6   

PF:GL1_4B6RNAi_reagentsjj_F09G2.4
MethodRNAi
WormBaseIDWBRNAi00007835
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits Predicted_geneF09G2.4 Source_evidenceePCR1066WS150 Sequence/Annotation Release
Gene F09G2.4 Source_evidenceePCR1066WS150 Sequence/Annotation Release
Map_statusUnique_ePCRV71825437183721
TranscriptF09G2.4 Source_evidenceePCR1066WS150 Sequence/Annotation Release
WB_Predicted_geneF09G2.4 Inferred_automaticallyRNAi_primary
WB_GeneF09G2.4 Inferred_automaticallyRNAi_primary
Gene_target F09G2.4 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolF09G2:RNAi
WB_Uniquely_mapped
Supporting_data Movie (3)
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype Cytokinesis defect
Delayed P0 spindle rotation
Emb Remark% penetrance
Penetrance100
Excessive blebbing
Large cytoplasmic granules
Multiple nuclei in 1, 2, or 4-cell embryos
P0 spindle does not rotate
Pronuclei meet more centrally
Phenotypic_signature Discrete_pheno_char 1No
2No
3No
4No
5No
6Yes
7Yes
8No
9No
10No
11No
12Unsure
13Unsure
14No
15No
16No
17No
18No
19No
20No
21No
22No
23No
24No
25Yes
26No
27No
28Yes
29Yes
30No
31Yes
32Yes
33No
34No
35No
36No
37No
38No
39No
40No
41No
42No
43No
44No
45No
46No
47No
Phenotype_summary (7)