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RNAi: PF:GL1_4D12 
| PF:GL1_4D12 | RNAi_reagent | sjj_F55H2.4 | ||||||
|---|---|---|---|---|---|---|---|---|
| Method | RNAi | |||||||
| WormBaseID | WBRNAi00007854 | |||||||
| Gene_set | GL1 | |||||||
| Reference | WBPaper00005599 | |||||||
| Experiment (2) | ||||||||
| Inhibits | Pseudogene | F55H2.4 | Source_evidence | ePCR | 1119 | WS150 Sequence/Annotation Release | ||
| Map_status | Unique_ePCR | III | 9504806 | 9505971 | ||||
| WB_Predicted_gene | K08E7.3 | Inferred_automatically | RNAi_secondary | |||||
| WB_Gene | F55H2.4 | Inferred_automatically | RNAi_primary | |||||
| K08E7.3 | Inferred_automatically | RNAi_secondary | ||||||
| WB_Pseudogene | F55H2.4 | Inferred_automatically | RNAi_primary | |||||
| Gene_target | F55H2.4 | RNAiDB | ePCR | ePCR_unique | ||||
| Wormbase | BLAST | BLAST_wormbase_primary | ||||||
| WB | BLAST_wormbase_primary | |||||||
| K08E7.3 | Wormbase | BLAST | BLAST_wormbase_secondary | |||||
| WB_Homol | Homol_homol | K08E7:RNAi | ||||||
| F55H2:RNAi | ||||||||
| Supporting_data | Movie | 4d12-02.mov | ||||||
| 4d12-0101.mov | ||||||||
| Remark | PCR product contains flanking T7 promoter sequences | |||||||
| Delivery | Delivered_by | Injection | ||||||
| Results | Phenotype | Aberrant cell-cell contacts at the four-cell stage | ||||||
| Delayed P0 spindle rotation | ||||||||
| Division axis of ABa or ABp aberrant | ||||||||
| Emb | Remark | % penetrance range | ||||||
| Penetrance | 80 | 100 | ||||||
| Large cytoplasmic granules | ||||||||
| Lva | Remark | Observed in >=10% of progeny. | ||||||
| P0 spindle does not rotate | ||||||||
| P0 spindle positioning defect | ||||||||
| Pronuclei meet more centrally | ||||||||
| Stp | Remark | Observed in >=10% of progeny. | ||||||
| Synchronous second division | ||||||||
| Phenotypic_signature | Discrete_pheno_char (47) | |||||||
| Phenotype_summary (7) |
