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RNAi: PF:GL1_4E2   

PF:GL1_4E2RNAi_reagentsjj_AH6.5
MethodRNAi
WormBaseIDWBRNAi00007867
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits Predicted_geneAH6.5 Source_evidenceePCR710WS150 Sequence/Annotation Release
Gene AH6.5 Source_evidenceePCR729WS150 Sequence/Annotation Release
Map_statusUnique_ePCRII95248889525807
TranscriptAH6.5 Source_evidenceePCR729WS150 Sequence/Annotation Release
WB_Predicted_gene AH6.5 Inferred_automaticallyRNAi_primary
W02A2.7 Inferred_automaticallyRNAi_secondary
WB_Gene AH6.5 Inferred_automaticallyRNAi_primary
W02A2.7 Inferred_automaticallyRNAi_secondary
Gene_target AH6.5 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
W02A2.7 WormbaseBLASTBLAST_wormbase_secondary
WB_Homol Homol_homol W02A2:RNAi
AH6:RNAi
Supporting_data Movie 4e2-03.mov
4e2-0101.mov
4e2-0201.mov
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Division axis of ABa or ABp aberrant
Emb Remark% penetrance
Penetrance100
EMS fails to extend anteriorly
P0 spindle positioning defect
P2 and EMS divide synchronously
Synchronous second division
Phenotypic_signature Discrete_pheno_char (47)
Phenotype_summary (7)