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RNAi: PF:GL1_4F2   

PF:GL1_4F2RNAi_reagentsjj_T10B5.6
MethodRNAi
WormBaseIDWBRNAi00007877
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits Predicted_geneT10B5.6 Source_evidenceePCR855WS150 Sequence/Annotation Release
Gene T10B5.6 Source_evidenceePCR855WS150 Sequence/Annotation Release
Map_statusUnique_ePCRV18691061870076
TranscriptT10B5.6 Source_evidenceePCR855WS150 Sequence/Annotation Release
WB_Predicted_geneT10B5.6 Inferred_automaticallyRNAi_primary
WB_GeneT10B5.6 Inferred_automaticallyRNAi_primary
Gene_target T10B5.6 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolT10B5:RNAi
WB_Uniquely_mapped
Supporting_data Movie 4f2-01.mov
4f2-0401.mov
4f2-0501.mov
4f2-0601.mov
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype Abnormal size of pronucleus
Cytokinesis defect
Emb Remark% penetrance
Penetrance100
Excessive blebbing
Large cytoplasmic granules
Multiple cavities
Multiple maternal pronuclei
Multiple nuclei in 1, 2, or 4-cell embryos
Nuclei reform next to cell division remnant
P0 spindle does not rotate
Phenotypic_signature Discrete_pheno_char (47)
Phenotype_summary (7)