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RNAi: PF:GL1_5E2   

PF:GL1_5E2RNAi_reagentsjj_C39E9.13
MethodRNAi
WormBaseIDWBRNAi00007957
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits Predicted_geneC39E9.13 Source_evidenceePCR772WS150 Sequence/Annotation Release
Gene C39E9.13 Source_evidenceePCR831WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIV1309607613097047
TranscriptC39E9.13 Source_evidenceePCR831WS150 Sequence/Annotation Release
WB_Predicted_geneC39E9.13 Inferred_automaticallyRNAi_primary
WB_GeneC39E9.13 Inferred_automaticallyRNAi_primary
Gene_target C39E9.13 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolC39E9:RNAi
WB_Uniquely_mapped
Supporting_data Movie 5e2-01.mov
5e2-0301.mov
Remark PCR product contains flanking T7 promoter sequences
Original annotation also included phenotype Mel, with remark 'Observed in >=10 of progeny'.
DeliveryDelivered_byInjection
Results Phenotype Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Division axis of ABa or ABp aberrant
Ectopic cleavage furrows
Emb Remark% penetrance range
Penetrance80100
EMS fails to extend anteriorly
Exaggerated asynchrony
Excessive blebbing
Large cytoplasmic granules
Multiple cavities
Nuclei reform next to cell division remnant
P0 spindle positioning defect
P2 and EMS divide synchronously
Phenotypic_signature Discrete_pheno_char 1No
2No
3No
4No
5No
6Yes
7Yes
8Yes
9Yes
10No
11No
12No
13No
14No
15No
16No
17No
18No
19No
20No
21No
22No
23No
24No
25No
26Yes
27No
28No
29No
30No
31No
32No
33No
34Yes
35No
36No
37Yes
38Yes
39No
40No
41Yes
42No
43No
44Yes
45Yes
46Yes
47No
Phenotype_summary Ste ResultNo
ReasonNone_detected
Stp ResultNo
ReasonNone_detected
EmbResultYes
EmbPhenoResultYes
Emb_image_dataResultYes
PostembPhenoResultYes
Movies_imagesResultYes