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RNAi: PF:GL1_6E2   

PF:GL1_6E2RNAi_reagentsjj_F30F8.8
MethodRNAi
WormBaseIDWBRNAi00008050
Gene_setGL1
ReferenceWBPaper00005599
Experiment (2)
Inhibits Gene F30F8.8 Source_evidenceePCR786WS150 Sequence/Annotation Release
Map_statusUnique_ePCRI78519917853184
Transcript F30F8.8.1 Source_evidenceePCR786WS150 Sequence/Annotation Release
F30F8.8.2 Source_evidenceePCR786WS150 Sequence/Annotation Release
F30F8.8.3 Source_evidenceePCR786WS150 Sequence/Annotation Release
WB_Predicted_geneF30F8.8 Inferred_automaticallyRNAi_primary
WB_GeneF30F8.8 Inferred_automaticallyRNAi_primary
Gene_target (4)
WB_HomolHomol_homolR11A5:RNAi
WB_Uniquely_mapped
Supporting_data Movie 6e2-08.mov
6e2-0201.mov
6e2-0301.mov
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype Cytokinesis defect
Emb (2)
Exaggerated pseudocleavage
Excessive blebbing
Large cytoplasmic granules
Loose mitotic furrow
Multiple nuclei in 1, 2, or 4-cell embryos
P0 spindle positioning defect
Polar bodies abnormal
Sck RemarkObserved in >=10% of progeny.
Synchronous second division
Phenotypic_signature Discrete_pheno_char 1No
2No
3No
4No
5No
6Yes
7Yes
8No
9No
10No
11No
12No
13Yes
14No
15Yes
16No
17No
18No
19No
20No
21No
22No
23No
24No
25No
26Yes
27No
28No
29No
30Yes
31Yes
32Yes
33No
34No
35No
36Yes
37Not applicable
38Not applicable
39No
40No
41Not applicable
42Not applicable
43Not applicable
44Not applicable
45No
46Not applicable
47No
Phenotype_summary (7)