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RNAi: PF:GL1_7D2 
| PF:GL1_7D2 | RNAi_reagent | sjj_C37A2.4 | ||||
|---|---|---|---|---|---|---|
| Method | RNAi | |||||
| WormBaseID | WBRNAi00008132 | |||||
| Gene_set | GL1 | |||||
| Reference | WBPaper00005599 | |||||
| Experiment | Laboratory | KK | ||||
| Date | 19 Nov 2002 00:00:00 | |||||
| Inhibits (6) | ||||||
| WB_Homol | Homol_homol | C37A2:RNAi | ||||
| WB_Uniquely_mapped | ||||||
| Supporting_data | Movie | 7d2-02.mov | ||||
| 7d2-03.mov | ||||||
| 7d2-06.mov | ||||||
| 7d2-0101.mov | ||||||
| 7d2-0401.mov | ||||||
| Remark | PCR product contains flanking T7 promoter sequences | |||||
| Delivery | Delivered_by | Injection | ||||
| Results | Phenotype | ABa, ABp and EMS divide synchronously | ||||
| Aberrant AB or P1 spindle orientation | ||||||
| Abnormal size of pronucleus | ||||||
| Emb | Remark | % penetrance range | ||||
| Penetrance | 80 | 100 | ||||
| EMS fails to extend anteriorly | ||||||
| Lva | Remark | Observed in >=10% of progeny. | ||||
| Multiple cavities | ||||||
| Multiple maternal pronuclei | ||||||
| Multiple nuclei in 1, 2, or 4-cell embryos | ||||||
| P0 spindle positioning defect | ||||||
| P1 aster abnormal | ||||||
| P2 and EMS divide synchronously | ||||||
| Pronuclei meet more centrally | ||||||
| Pseudocleavage absent | ||||||
| Stp | Remark | Observed in >=10% of progeny. | ||||
| Synchronous second division | ||||||
| Pleiotropic | ||||||
| Phenotypic_signature | Discrete_pheno_char (47) | |||||
| Phenotype_summary (7) |
