RNAi: PF:GL1_7F2
PF:GL1_7F2 | RNAi_reagent | sjj_ZK1127.5 | ||||||
---|---|---|---|---|---|---|---|---|
Method | RNAi | |||||||
WormBaseID | WBRNAi00008154 | |||||||
Gene_set | GL1 | |||||||
Reference | WBPaper00005599 | |||||||
Experiment (2) | ||||||||
Inhibits | Predicted_gene | ZK1127.5 | Source_evidence | ePCR | 1037 | WS150 Sequence/Annotation Release | ||
Gene | ZK1127.5 | Source_evidence | ePCR | 1037 | WS150 Sequence/Annotation Release | |||
Map_status | Unique_ePCR | II | 7050677 | 7051807 | ||||
Transcript | ZK1127.5 | Source_evidence | ePCR | 1037 | WS150 Sequence/Annotation Release | |||
WB_Predicted_gene | ZK1127.5 | Inferred_automatically | RNAi_primary | |||||
WB_Gene | ZK1127.5 | Inferred_automatically | RNAi_primary | |||||
Gene_target | ZK1127.5 | RNAiDB | ePCR | ePCR_unique | ||||
Wormbase | BLAST | BLAST_wormbase_primary | ||||||
WB_Homol | Homol_homol | ZK1127:RNAi | ||||||
WB_Uniquely_mapped | ||||||||
Supporting_data | Movie | 7f2-0101.mov | ||||||
Remark | PCR product contains flanking T7 promoter sequences | |||||||
Delivery | Delivered_by | Injection | ||||||
Results | Phenotype | Cytokinesis defect | ||||||
Delayed P0 spindle rotation | ||||||||
Dpy | Remark | Observed in >=10% of progeny. | ||||||
Ectopic cleavage furrows | ||||||||
Egl | Remark | Observed in <10% of progeny. | ||||||
Emb | Remark | % penetrance range | ||||||
Penetrance | 80 | 100 | ||||||
Excessive blebbing | ||||||||
Gro | Remark | Observed in >=10% of progeny. | ||||||
Lva | Remark | Observed in >=10% of progeny. | ||||||
Multiple nuclei in 1, 2, or 4-cell embryos | ||||||||
P0 spindle does not rotate | ||||||||
P0 spindle positioning defect | ||||||||
Pronuclei meet more centrally | ||||||||
Pseudocleavage absent | ||||||||
Pvl | Remark | Observed in >=10% of progeny. | ||||||
Slow cell cycle | ||||||||
Stp | Remark | Observed in >=10% of progeny. | ||||||
no escapers | ||||||||
Phenotypic_signature | Discrete_pheno_char (47) | |||||||
Phenotype_summary (7) |