RNAi: PF:GL1_8B9
PF:GL1_8B9 | RNAi_reagent | sjj_F22B7.5 | ||||||
---|---|---|---|---|---|---|---|---|
Method | RNAi | |||||||
WormBaseID | WBRNAi00008207 | |||||||
Gene_set | GL1 | |||||||
Reference | WBPaper00005599 | |||||||
Experiment | Laboratory | KK | ||||||
Date | 19 Nov 2002 00:00:00 | |||||||
Inhibits | Predicted_gene | C38C10.4 | Source_evidence | ePCR | 1059 | WS150 Sequence/Annotation Release | ||
F22B7.13 | Source_evidence | ePCR | 1059 | WS150 Sequence/Annotation Release | ||||
Gene | C38C10.4 | Source_evidence | ePCR | 1059 | WS150 Sequence/Annotation Release | |||
Map_status | Best_ePCR | III | 9391789 | 9392950 | ||||
F22B7.13 | Source_evidence | ePCR | 1059 | WS150 Sequence/Annotation Release | ||||
Map_status | Best_ePCR | III | 8628947 | 8630106 | ||||
Transcript | C38C10.4 | Source_evidence | ePCR | 1059 | WS150 Sequence/Annotation Release | |||
F22B7.13 | Source_evidence | ePCR | 1059 | WS150 Sequence/Annotation Release | ||||
WB_Predicted_gene | C38C10.4 | Inferred_automatically | RNAi_primary | |||||
F22B7.13 | Inferred_automatically | RNAi_primary | ||||||
WB_Gene | C38C10.4 | Inferred_automatically | RNAi_primary | |||||
F22B7.13 | Inferred_automatically | RNAi_primary | ||||||
Gene_target | C38C10.4 | RNAiDB | ePCR | ePCR_best | ||||
Wormbase | BLAST | BLAST_wormbase_primary | ||||||
F22B7.13 | RNAiDB | ePCR | ePCR_best | |||||
Wormbase | BLAST | BLAST_wormbase_primary | ||||||
WB_Homol | Homol_homol | F22B7:RNAi | ||||||
C38C10:RNAi | ||||||||
Supporting_data | Movie | 8b9-04.mov | ||||||
8b9-0201.mov | ||||||||
8b9-0301.mov | ||||||||
Remark | PCR product contains flanking T7 promoter sequences | |||||||
Delivery | Delivered_by | Injection | ||||||
Results | Phenotype | ABa, ABp and EMS divide synchronously | ||||||
Aberrant AB or P1 spindle orientation | ||||||||
Division axis of ABa or ABp aberrant | ||||||||
Emb | Remark | % penetrance range | ||||||
Penetrance | 80 | 100 | ||||||
Him | Remark | >0.4% male progeny observed. | ||||||
Multiple nuclei in 1, 2, or 4-cell embryos | ||||||||
Nuclei reform next to cell division remnant | ||||||||
P0 spindle positioning defect | ||||||||
P1 aster abnormal | ||||||||
Pronuclei meet more centrally | ||||||||
Synchronous second division | ||||||||
Phenotypic_signature | Discrete_pheno_char (47) | |||||||
Phenotype_summary (7) |