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RNAi: PF:GL1_8D2   

PF:GL1_8D2RNAi_reagentsjj_H02I12.8
MethodRNAi
WormBaseIDWBRNAi00008224
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits Predicted_geneH02I12.8 Source_evidenceePCR943WS150 Sequence/Annotation Release
Gene H02I12.8 Source_evidenceePCR943WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIV1140394911405074
TranscriptH02I12.8 Source_evidenceePCR943WS150 Sequence/Annotation Release
WB_Predicted_gene H02I12.8 Inferred_automaticallyRNAi_primary
Y17G9B.3 Inferred_automaticallyRNAi_secondary
WB_Gene C01F6.3 Inferred_automaticallyRNAi_secondary
H02I12.8 Inferred_automaticallyRNAi_primary
Y17G9B.3 Inferred_automaticallyRNAi_secondary
Y62E10A.15 Inferred_automaticallyRNAi_primary
WB_Pseudogene C01F6.3 Inferred_automaticallyRNAi_secondary
Y62E10A.15 Inferred_automaticallyRNAi_primary
Gene_target H02I12.8 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
Y17G9B.3 WormbaseBLASTBLAST_wormbase_secondary
C01F6.3 WormbaseBLASTBLAST_wormbase_secondary
WBBLAST_wormbase_secondary
Y62E10A.15 WormbaseBLASTBLAST_wormbase_primary
WBBLAST_wormbase_primary
WB_Homol Homol_homol C01F6:RNAi
Y62E10A:RNAi
Y17G9B:RNAi
H02I12:RNAi
Supporting_data Movie (6)
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype AB aster resembles P1 aster
ABa, ABp and EMS divide synchronously
Aberrant AB or P1 spindle orientation
Delayed P0 spindle rotation
Division axis of ABa or ABp aberrant
Emb Remark% penetrance range
Penetrance80100
EMS fails to extend anteriorly
Multiple nuclei in 1, 2, or 4-cell embryos
Osmotically/ pressure sensitive
P0 spindle positioning defect
P2 and EMS divide synchronously
Polar body reabsorbed
Pseudocleavage absent
Synchronous second division
Phenotypic_signature Discrete_pheno_char (47)
Phenotype_summary (7)