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Gene: T09A5.10   

T09A5.10EvidenceCGC_data_submission
SMapS_parentSequenceT09A5
ChromosomeII
Identity (5)
Gene_info Gene_classlin
Reference_allelee1348
Allele (5)
Phenotype See also e1348, e1457
[C.elegansII] e1348 : thin, sterile and uncoordinated after L1; all postembryonic divisions fail from defective cytokinesis although DNA replication continues. No sexual maturation in either sex. ES3 ME0. OA1: e1457 (similar phenotypes). Cloned: cosmid rescue (C03G3)[Sulston and Horvitz 1981; Albertson et al. 1978; MT]
Strain (6)
Structured_description Provisional_descriptionlin-5 encodes a novel protein with a centrally located alpha-helical coiled-coil domain and ten potential proline-directed kinase phosphorylation sites; LIN-5 activity is required for mitosis and cytokinesis at all stages of development, specifically for spindle positioning, chromosome alignment and segregation, and perhaps also for blocking exit from mitosis when chromosome segregation fails; LIN-5, which is expressed maternally, embryonically, and during larval stages, localizes to the cell periphery and, in microtubule-dependent manner, to the meiotic spindle and the asters and kinetochore microtubules of the mitotic spindle; in regulating spindle function, LIN-5 interacts with two G protein regulators, GPR-1 and GPR-2, which require LIN-5 activity for localization to the spindle and cell cortex; additionally, LIN-5 interacts in vitro with itself, the coiled-coil protein LFI-1, GEI-16, and several other novel proteins; genetic studies indicate that in regulating embryonic spindle function, lin-5 acts downsteam of par-2, par-3, mes-1, and src-1 and upstream of gpr-1, gpr-2, goa-1, and gpa-16. Paper_evidence WBPaper00003888
WBPaper00005878
WBPaper00024244
Person_evidenceWBPerson1843
Curator_confirmedWBPerson1843
Date_last_updated09 Sep 2004 00:00:00
Concise_descriptionlin-5 encodes a novel protein with a centrally located alpha-helical coiled-coil domain and ten potential proline-directed kinase phosphorylation sites; LIN-5 activity is required for mitosis and cytokinesis at all stages of development, specifically for spindle positioning, chromosome alignment and segregation, and perhaps also for blocking exit from mitosis when chromosome segregation fails; LIN-5, which is expressed maternally, embryonically, and during larval stages, localizes to the cell periphery and, in microtubule-dependent manner, to the meiotic spindle and the asters and kinetochore microtubules of the mitotic spindle; in regulating spindle function, LIN-5 interacts with two G protein regulators, GPR-1 and GPR-2, which require LIN-5 activity for localization to the spindle and cell cortex; additionally, LIN-5 interacts in vitro with itself, the coiled-coil protein LFI-1, GEI-16, and several other novel proteins; genetic studies indicate that in regulating embryonic spindle function, lin-5 acts downsteam of par-2, par-3, mes-1, and src-1 and upstream of gpr-1, gpr-2, goa-1, and gpa-16.
Molecular_info (5)
Experimental_info (5)
Reference (48)
RemarkSequence connection from [Lorson MA, van den Heuvel S]
MethodGene