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RNAi: TH:300b9

TH:300b9	RNAi_reagent	TH:300b9
	Method	RNAi
	WormBaseID	WBRNAi00002427
	Reference	WBPaper00004403
	Experiment	Laboratory	TH
		Author (21)
		Date	16 Nov 2000 00:00:00
	Inhibits	Gene	B0393.1	Source_evidence	ePCR	888	WS150 Sequence/Annotation Release
				Map_status	Best_ePCR	III	4752152	4754209
		Transcript	B0393.1.1	Source_evidence	ePCR	888	WS150 Sequence/Annotation Release
			B0393.1.2	Source_evidence	ePCR	836	WS150 Sequence/Annotation Release
		Potential_predicted_gene	B0393.8	Source_evidence	ePCR	137	WS150 Sequence/Annotation Release
		Potential_Gene	B0393.8	Source_evidence	ePCR	137	WS150 Sequence/Annotation Release
				Map_status	Bottom50_percentile_ePCR	III	4752152	4754209
		Potential_Transcript	B0393.8	Source_evidence	ePCR	137	WS150 Sequence/Annotation Release
		WB_Predicted_gene	B0393.1	Inferred_automatically	RNAi_primary
			B0393.8	Inferred_automatically	RNAi_primary
		WB_Gene	B0393.1	Inferred_automatically	RNAi_primary
			B0393.8	Inferred_automatically	RNAi_primary
		Gene_target	B0393.1	RNAiDB	ePCR	ePCR_best
				Wormbase	BLAST	BLAST_wormbase_primary
			B0393.1.1	RNAiDB	ePCR
			B0393.1.2	RNAiDB	ePCR
			B0393.8	RNAiDB	ePCR
				Wormbase	BLAST	BLAST_wormbase_primary
	WB_Homol	Homol_homol	B0393:RNAi
	WB_Uniquely_mapped
	Remark	Phenotype comment -- Semi-sterile. Phenotype confimed by independent dsRNA (B0393.1-RNA2; similar phenotype).
		DIC phenotype -- Multiple female pronuclei; irregular cytoplasmic appearance; aberrant pseudocleavage stage; spindle unstable during anaphase; karyomeres in daughter blastomeres; nuclei in AB are off-center for a while, nuclei in P1 stay close to posterior cortex for a while. A bit slow overall. Partial egg-osmotic-sensitive (eos).
		DIC phenotype comment -- Composite phenotype (deduced from partial observations in different embryos). Not all embryos show all aspects of the DIC phenotype.
		All embryos dead
	Results	Phenotype	Emb
			Ste
		Phenotype_summary (7)