TH:300c6 | RNAi_reagent | TH:300c6
|
| Method | RNAi
|
| WormBaseID | WBRNAi00002431
|
| Reference | WBPaper00004403
|
|
Experiment
| Laboratory | TH
|
| |
Author (21)
|
| | Date | 16 Nov 2000 00:00:00 |
|
Inhibits
| Gene |
C36E8.5
| Source_evidence | ePCR | 1511 | WS150 Sequence/Annotation Release |
| | | | Map_status | Unique_ePCR | III | 4015893 | 4017836 |
| |
Transcript
| C36E8.5.1
| Source_evidence | ePCR | 1511 | WS150 Sequence/Annotation Release |
| | | C36E8.5.2
| Source_evidence | ePCR | 1437 | WS150 Sequence/Annotation Release |
| | | C36E8.5.3
| Source_evidence | ePCR | 1358 | WS150 Sequence/Annotation Release |
| | WB_Predicted_gene | C36E8.5
| Inferred_automatically | RNAi_primary |
| | WB_Gene | C36E8.5
| Inferred_automatically | RNAi_primary |
| |
Gene_target
|
C36E8.5
| RNAiDB | ePCR | ePCR_unique |
| | | | Wormbase | BLAST | BLAST_wormbase_primary |
| | | C36E8.5.1
| RNAiDB | ePCR |
| | | C36E8.5.2
| RNAiDB | ePCR |
| | | C36E8.5.3
| RNAiDB | ePCR |
| WB_Homol | Homol_homol | C36E8:RNAi
|
| WB_Uniquely_mapped |
|
Remark
| Phenotype comment -- Confirmed with independent dsRNA (C36E8.5-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for C36E8.5 function. |
| | DIC phenotype -- Lack of male pronuclear migration; female pronucleus is located initially towards center of embryo or next to male pronucleus; sometimes 2 female pronuclei; female pronuclei drift towards male pronucleus; no bipolar spindle assembly. |
| | All embryos dead |
|
Results
| Phenotype | Emb
|
| |
Phenotype_summary
|
Ste
| Result | No |
| | | | Reason | None_reported |
| | |
Stp
| Result | No |
| | | | Reason | None_reported |
| | | Emb | Result | Yes |
| | |
EmbPheno
| Result | No |
| | | | Reason | None_reported |
| | |
PostembLethal
| Result | No |
| | | | Reason | None_reported |
| | |
PostembPheno
| Result | No |
| | | | Reason | None_reported |
| | | Movies_images | Result | No |