RNAiDB Tree View

RNAi: TH:300c6

TH:300c6	RNAi_reagent	TH:300c6
	Method	RNAi
	WormBaseID	WBRNAi00002431
	Reference	WBPaper00004403
	Experiment	Laboratory	TH
		Author (21)
		Date	16 Nov 2000 00:00:00
	Inhibits	Gene	C36E8.5	Source_evidence	ePCR	1511	WS150 Sequence/Annotation Release
				Map_status	Unique_ePCR	III	4015893	4017836
		Transcript	C36E8.5.1	Source_evidence	ePCR	1511	WS150 Sequence/Annotation Release
			C36E8.5.2	Source_evidence	ePCR	1437	WS150 Sequence/Annotation Release
			C36E8.5.3	Source_evidence	ePCR	1358	WS150 Sequence/Annotation Release
		WB_Predicted_gene	C36E8.5	Inferred_automatically	RNAi_primary
		WB_Gene	C36E8.5	Inferred_automatically	RNAi_primary
		Gene_target (4)
	WB_Homol	Homol_homol	C36E8:RNAi
	WB_Uniquely_mapped
	Remark	Phenotype comment -- Confirmed with independent dsRNA (C36E8.5-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for C36E8.5 function.
		DIC phenotype -- Lack of male pronuclear migration; female pronucleus is located initially towards center of embryo or next to male pronucleus; sometimes 2 female pronuclei; female pronuclei drift towards male pronucleus; no bipolar spindle assembly.
		All embryos dead
	Results	Phenotype	Emb
		Phenotype_summary (7)