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RNAi: TH:300e8   

TH:300e8RNAi_reagentTH:300e8
MethodRNAi
WormBaseIDWBRNAi00002436
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneF35G12.10 Source_evidenceePCR798WS150 Sequence/Annotation Release
Gene F35G12.10 Source_evidenceePCR882WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIII45945444595631
TranscriptF35G12.10 Source_evidenceePCR882WS150 Sequence/Annotation Release
WB_Predicted_geneF35G12.10 Inferred_automaticallyRNAi_primary
WB_GeneF35G12.10 Inferred_automaticallyRNAi_primary
Gene_target F35G12.10 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolF35G12:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA (F35G12.10-RNA2; similar DIC phenotype, but progeny test was not embryonic lethal).
DIC phenotype -- Overall pace of development is slow (30 minutes or more between pronuclear migration and division of AB daughter blastomere -compared to 18-22 minutes in wild-type). Muliple female pronuclei in occasional embryos.
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)