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RNAi: TH:302c4   

TH:302c4RNAi_reagentTH:302c4
MethodRNAi
WormBaseIDWBRNAi00002460
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneF57B9.3 Source_evidenceePCR1001WS150 Sequence/Annotation Release
Gene F57B9.3 Source_evidenceePCR1001WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIII69401996941729
TranscriptF57B9.3 Source_evidenceePCR1001WS150 Sequence/Annotation Release
WB_Predicted_geneF57B9.3 Inferred_automaticallyRNAi_primary
WB_GeneF57B9.3 Inferred_automaticallyRNAi_primary
Gene_target F57B9.3 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolF57B9:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Semi-sterile
DIC phenotype -- Multiple female pronuclei; irregular cytoplasmic appearance; aberrant pseudocleavage stage; spindle unstable during anaphase; karyomeres in daughter blastomeres; nuclei in AB are off-center for a while, nuclei in P1 stay close to posterior cortex for a while. A bit slow overall.
DIC phenotype comment -- Placed in fidelity of meiotic division phenotypic class because shares most phenotypic manifestations typical of this class -although multiple pronuclei were observed in only 1/10 embryos.
All embryos dead
Results Phenotype Emb
Ste
Phenotype_summary (7)