TH:302f10 | RNAi_reagent | TH:302f10
|
| Method | RNAi
|
| WormBaseID | WBRNAi00002467
|
| Reference | WBPaper00004403
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Experiment
| Laboratory | TH
|
| |
Author (21)
|
| | Date | 16 Nov 2000 00:00:00 |
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Inhibits
| Predicted_gene | C14B9.4b
| Source_evidence | ePCR | 1058 | WS150 Sequence/Annotation Release |
| | Gene |
C14B9.4
| Source_evidence | ePCR | 1407 | WS150 Sequence/Annotation Release |
| | | | Map_status | Unique_ePCR | III | 8102448 | 8104613 |
| |
Transcript
| C14B9.4a.1
| Source_evidence | ePCR | 1402 | WS150 Sequence/Annotation Release |
| | | C14B9.4a.2
| Source_evidence | ePCR | 1399 | WS150 Sequence/Annotation Release |
| | | C14B9.4b
| Source_evidence | ePCR | 1407 | WS150 Sequence/Annotation Release |
| |
WB_Predicted_gene
| C14B9.4a
| Inferred_automatically | RNAi_primary |
| | | C14B9.4b
| Inferred_automatically | RNAi_primary |
| | WB_Gene | C14B9.4
| Inferred_automatically | RNAi_primary |
| |
Gene_target (5)
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| WB_Homol | Homol_homol | C14B9:RNAi
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| WB_Uniquely_mapped |
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Remark
| Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction. |
| | DIC phenotype -- Complex phenotype. In most embryos (e.g. movie1), eggshell appears fragile as witnessed by random yolk granule movements movements; pronuclei appear present, but fragility of embryos prevents thorough phenotypic characterization; no progress to the two cell stage; in other embryos (e.g. movie 2), defect in spindle assembly; also, no visible cleavage furrow; often large cytoplast on anterior side |
| | DIC phenotype comment -- See also phenotypic description in Chase et al. (2000). |
| | All embryos dead |
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Results
| Phenotype | Emb
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Phenotype_summary (7)
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