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RNAi: TH:302f10   

TH:302f10RNAi_reagentTH:302f10
MethodRNAi
WormBaseIDWBRNAi00002467
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneC14B9.4b Source_evidenceePCR1058WS150 Sequence/Annotation Release
Gene C14B9.4 Source_evidenceePCR1407WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIII81024488104613
Transcript C14B9.4a.1 Source_evidenceePCR1402WS150 Sequence/Annotation Release
C14B9.4a.2 Source_evidenceePCR1399WS150 Sequence/Annotation Release
C14B9.4b Source_evidenceePCR1407WS150 Sequence/Annotation Release
WB_Predicted_gene C14B9.4a Inferred_automaticallyRNAi_primary
C14B9.4b Inferred_automaticallyRNAi_primary
WB_GeneC14B9.4 Inferred_automaticallyRNAi_primary
Gene_target (5)
WB_HomolHomol_homolC14B9:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction.
DIC phenotype -- Complex phenotype. In most embryos (e.g. movie1), eggshell appears fragile as witnessed by random yolk granule movements movements; pronuclei appear present, but fragility of embryos prevents thorough phenotypic characterization; no progress to the two cell stage; in other embryos (e.g. movie 2), defect in spindle assembly; also, no visible cleavage furrow; often large cytoplast on anterior side
DIC phenotype comment -- See also phenotypic description in Chase et al. (2000).
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)