RNAi: TH:302h12
TH:302h12 | RNAi_reagent | TH:302h12 | |
---|---|---|---|
Method | RNAi | ||
WormBaseID | WBRNAi00002472 | ||
Reference | WBPaper00004403 | ||
Experiment (3) | |||
Inhibits (5) | |||
WB_Homol | Homol_homol | C30C11:RNAi | |
WB_Uniquely_mapped | |||
Remark | Phenotype comment -- Confirmed with independent dsRNA (C30C11.2-RNA2; similar phenotype). | ||
DIC phenotype -- In most embryos (e.g. movie1), the male and female pronuclei never become visible, and everything seems to be arrested. Some embryos progressed to the one cell stage (e.g. movie2): in these cases, there is a sriking delay during mitosis (over 20 min between breakdown of the pronuclear envelopes and cleavage furrow ingression, compared to the usual approximately 2 min in wild-type); earlier events in the one cell stage may be slow as well. | |||
DIC phenotype comment -- Staining of fixed embryos with DNA dye shows that fertilization took place, and suggests that at least half of the embryos are arrested during meiotic divisions. | |||
All embryos dead | |||
Results | Phenotype | Emb | |
Phenotype_summary (7) |