TH:311f4 | RNAi_reagent | TH:311f4
|
| Method | RNAi
|
| WormBaseID | WBRNAi00002542
|
| Reference | WBPaper00004403
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Experiment (3)
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Inhibits
| Predicted_gene | F35G12.9
| Source_evidence | ePCR | 372 | WS150 Sequence/Annotation Release |
| | Gene |
F35G12.9
| Source_evidence | ePCR | 522 | WS150 Sequence/Annotation Release |
| | | | Map_status | Best_ePCR | III | 4593661 | 4594351 |
| | Transcript | F35G12.9
| Source_evidence | ePCR | 522 | WS150 Sequence/Annotation Release |
| | Potential_predicted_gene | F35G12.11
| Source_evidence | ePCR | 132 | WS150 Sequence/Annotation Release |
| | Potential_Gene |
F35G12.11 (2)
|
| |
Potential_Transcript
| F35G12.11.1
| Source_evidence | ePCR | 117 | WS150 Sequence/Annotation Release |
| | | F35G12.11.2
| Source_evidence | ePCR | 132 | WS150 Sequence/Annotation Release |
| | WB_Predicted_gene | F35G12.9
| Inferred_automatically | RNAi_primary |
| | WB_Gene | F35G12.9
| Inferred_automatically | RNAi_primary |
| |
Gene_target
|
F35G12.9
| RNAiDB | ePCR | ePCR_best |
| | | | Wormbase | BLAST | BLAST_wormbase_primary |
| | | F35G12.11
| RNAiDB | ePCR |
| | | F35G12.11.1
| RNAiDB | ePCR |
| | | F35G12.11.2
| RNAiDB | ePCR |
| WB_Homol | Homol_homol | F35G12:RNAi
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| WB_Uniquely_mapped |
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Remark
| Phenotype comment -- Confirmed with independent dsRNA (F35G12.9-RNA2; similar -albeit seemingly weaker- phenotype). |
| | DIC phenotype -- Eggshell appears fragile in all embryos as witnessed by random yolk granule movements movements and absence of space between embryo proper and eggshell; in some embryos (e.g. movie1), there are no signs of progress into the first cell cycle; in other embryos (e.g. movie2) early events ensue, but there is a striking delay during mitosis (over 10 min between breakdown of the pronuclear envelopes and cleavage furrow ingression, compared to the usual approximately 2 min in wild-type) |
| | All embryos dead |
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Results (2)
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