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RNAi: TH:311f4   

TH:311f4RNAi_reagentTH:311f4
MethodRNAi
WormBaseIDWBRNAi00002542
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author Gonczy P
Echeverri C
Oegema K
Coulson AR
Jones SJ
Copley RR
Duperon J
Oegema J
Brehm M
Cassin E
Hannak E
Kirkham M
Pichler SC
Flohrs K
Go essen A
Leidel S
Alleaume AM
Martin C
Ozlu N
Bork P
Hyman AA
Date16 Nov 2000 00:00:00
Inhibits (9)
WB_HomolHomol_homolF35G12:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA (F35G12.9-RNA2; similar -albeit seemingly weaker- phenotype).
DIC phenotype -- Eggshell appears fragile in all embryos as witnessed by random yolk granule movements movements and absence of space between embryo proper and eggshell; in some embryos (e.g. movie1), there are no signs of progress into the first cell cycle; in other embryos (e.g. movie2) early events ensue, but there is a striking delay during mitosis (over 10 min between breakdown of the pronuclear envelopes and cleavage furrow ingression, compared to the usual approximately 2 min in wild-type)
All embryos dead
Results PhenotypeEmb
Phenotype_summary Ste ResultNo
ReasonNone_reported
Stp ResultNo
ReasonNone_reported
EmbResultYes
EmbPheno (2)
PostembLethal (2)
PostembPheno ResultNo
ReasonNone_reported
Movies_imagesResultNo