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RNAi: TH:311f4   

Experiment LaboratoryTH
Author Gonczy P
Echeverri C
Oegema K
Coulson AR
Jones SJ
Copley RR
Duperon J
Oegema J
Brehm M
Cassin E
Hannak E
Kirkham M
Pichler SC
Flohrs K
Go essen A
Leidel S
Alleaume AM
Martin C
Ozlu N
Bork P
Hyman AA
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneF35G12.9 Source_evidenceePCR372WS150 Sequence/Annotation Release
Gene F35G12.9 Source_evidenceePCR522WS150 Sequence/Annotation Release
TranscriptF35G12.9 Source_evidenceePCR522WS150 Sequence/Annotation Release
Potential_predicted_geneF35G12.11 Source_evidenceePCR132WS150 Sequence/Annotation Release
Potential_Gene F35G12.11 Source_evidenceePCR132WS150 Sequence/Annotation Release
Potential_Transcript F35G12.11.1 Source_evidenceePCR117WS150 Sequence/Annotation Release
F35G12.11.2 Source_evidenceePCR132WS150 Sequence/Annotation Release
WB_Predicted_geneF35G12.9 Inferred_automaticallyRNAi_primary
WB_GeneF35G12.9 Inferred_automaticallyRNAi_primary
Gene_target F35G12.9 RNAiDBePCRePCR_best
F35G12.11 RNAiDBePCR
F35G12.11.1 RNAiDBePCR
F35G12.11.2 RNAiDBePCR
Remark Phenotype comment -- Confirmed with independent dsRNA (F35G12.9-RNA2; similar -albeit seemingly weaker- phenotype).
DIC phenotype -- Eggshell appears fragile in all embryos as witnessed by random yolk granule movements movements and absence of space between embryo proper and eggshell; in some embryos (e.g. movie1), there are no signs of progress into the first cell cycle; in other embryos (e.g. movie2) early events ensue, but there is a striking delay during mitosis (over 10 min between breakdown of the pronuclear envelopes and cleavage furrow ingression, compared to the usual approximately 2 min in wild-type)
All embryos dead
Results (2)