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RNAi: TH:320e11   

TH:320e11RNAi_reagentTH:320e11
MethodRNAi
WormBaseIDWBRNAi00002583
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author Gonczy P
Echeverri C
Oegema K
Coulson AR
Jones SJ
Copley RR
Duperon J
Oegema J
Brehm M
Cassin E
Hannak E
Kirkham M
Pichler SC
Flohrs K
Go essen A
Leidel S
Alleaume AM
Martin C
Ozlu N
Bork P
Hyman AA
Date16 Nov 2000 00:00:00
Inhibits (5)
WB_HomolHomol_homolK01G5:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA (K01G5.7-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for K01G5.7 function.
DIC phenotype -- Lack of male pronuclear migration; female pronucleus is usually located initially towards center of embryo or next to male pronucleus; sometimes 2 female pronuclei; lack of female pronuclear migration (where applicable, i.e. in those embryos in which the female pronucleus is located away from the male pronucleus); no bipolar spindle assembly.
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)