RNAi: TH:320e11
TH:320e11 | RNAi_reagent | TH:320e11 | |
---|---|---|---|
Method | RNAi | ||
WormBaseID | WBRNAi00002583 | ||
Reference | WBPaper00004403 | ||
Experiment | Laboratory | TH | |
Author | Gonczy P | ||
Echeverri C | |||
Oegema K | |||
Coulson AR | |||
Jones SJ | |||
Copley RR | |||
Duperon J | |||
Oegema J | |||
Brehm M | |||
Cassin E | |||
Hannak E | |||
Kirkham M | |||
Pichler SC | |||
Flohrs K | |||
Go essen A | |||
Leidel S | |||
Alleaume AM | |||
Martin C | |||
Ozlu N | |||
Bork P | |||
Hyman AA | |||
Date | 16 Nov 2000 00:00:00 | ||
Inhibits (5) | |||
WB_Homol | Homol_homol | K01G5:RNAi | |
WB_Uniquely_mapped | |||
Remark | Phenotype comment -- Confirmed with independent dsRNA (K01G5.7-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for K01G5.7 function. | ||
DIC phenotype -- Lack of male pronuclear migration; female pronucleus is usually located initially towards center of embryo or next to male pronucleus; sometimes 2 female pronuclei; lack of female pronuclear migration (where applicable, i.e. in those embryos in which the female pronucleus is located away from the male pronucleus); no bipolar spindle assembly. | |||
All embryos dead | |||
Results | Phenotype | Emb | |
Phenotype_summary (7) |