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RNAi: TH:331a9   

TH:331a9RNAi_reagentTH:331a9
MethodRNAi
WormBaseIDWBRNAi00002666
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneY49E10.15 Source_evidenceePCR273WS150 Sequence/Annotation Release
Gene Y49E10.15 Source_evidenceePCR273WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIII1242915012430012
TranscriptY49E10.15 Source_evidenceePCR273WS150 Sequence/Annotation Release
WB_Predicted_geneY49E10.15 Inferred_automaticallyRNAi_primary
WB_GeneY49E10.15 Inferred_automaticallyRNAi_primary
Gene_target Y49E10.15 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolY49E10:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA (Y49E10.15-RNA2; similar phenotype).
DIC phenotype -- Sparse yolk granules and/or areas lacking yolk granules (especially at anterior of embryo); sometimes incomplete centration/rotation (yet, spindle adopts proper position by end of anaphase); separation between daughter blastomeres is less apparent than in wild-type (weak cortex?); some embryos have large polar body
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)