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RNAi: TH:335f2   

TH:335f2RNAi_reagentTH:335f2
MethodRNAi
WormBaseIDWBRNAi00002693
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneF58A4.14 Source_evidenceePCR417WS150 Sequence/Annotation Release
Gene F58A4.14 Source_evidenceePCR417WS150 Sequence/Annotation Release
Map_statusBest_ePCRIII96108539612505
TranscriptF58A4.14 Source_evidenceePCR417WS150 Sequence/Annotation Release
Potential_predicted_geneF58A4.4 Source_evidence ePCR 249WS150 Sequence/Annotation Release
216WS150 Sequence/Annotation Release
Potential_Gene F58A4.4 (2)
Potential_TranscriptF58A4.4 Source_evidenceePCR249WS150 Sequence/Annotation Release
WB_Predicted_gene F58A4.14 Inferred_automaticallyRNAi_primary
F58A4.4 Inferred_automaticallyRNAi_primary
WB_Gene F58A4.14 Inferred_automaticallyRNAi_primary
F58A4.4 Inferred_automaticallyRNAi_primary
Gene_target F58A4.14 RNAiDBePCRePCR_best
WormbaseBLASTBLAST_wormbase_primary
F58A4.4 (2)
WB_HomolHomol_homolF58A4:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA (F58A4.4 -RNA2; similar phenotype).
DIC phenotype -- Slow between pseudocleavage stage and pronuclear envelope breakdown (8 min or more compared to 4-5 min in wild-type); P1 late (5 min or more between division of AB and P1, compared to 2-3 min in wild-type); lack of rotation in P1.
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)