TH:335f2 | RNAi_reagent | TH:335f2
|
| Method | RNAi
|
| WormBaseID | WBRNAi00002693
|
| Reference | WBPaper00004403
|
|
Experiment (3)
|
|
Inhibits
| Predicted_gene | F58A4.14
| Source_evidence | ePCR | 417 | WS150 Sequence/Annotation Release |
| | Gene |
F58A4.14
| Source_evidence | ePCR | 417 | WS150 Sequence/Annotation Release |
| | | | Map_status | Best_ePCR | III | 9610853 | 9612505 |
| | Transcript | F58A4.14
| Source_evidence | ePCR | 417 | WS150 Sequence/Annotation Release |
| | Potential_predicted_gene | F58A4.4
| Source_evidence |
ePCR
| 249 | WS150 Sequence/Annotation Release |
| | | | | | 216 | WS150 Sequence/Annotation Release |
| | Potential_Gene |
F58A4.4
| Source_evidence | ePCR | 249 | WS150 Sequence/Annotation Release |
| | | | Map_status | Top50_percentile_ePCR | III | 9610853 | 9612505 |
| | Potential_Transcript | F58A4.4
| Source_evidence | ePCR | 249 | WS150 Sequence/Annotation Release |
| |
WB_Predicted_gene
| F58A4.14
| Inferred_automatically | RNAi_primary |
| | | F58A4.4
| Inferred_automatically | RNAi_primary |
| |
WB_Gene
| F58A4.14
| Inferred_automatically | RNAi_primary |
| | | F58A4.4
| Inferred_automatically | RNAi_primary |
| |
Gene_target
|
F58A4.14
| RNAiDB | ePCR | ePCR_best |
| | | | Wormbase | BLAST | BLAST_wormbase_primary |
| | |
F58A4.4
| RNAiDB | ePCR | ePCR_Top50 |
| | | | Wormbase | BLAST | BLAST_wormbase_primary |
| WB_Homol | Homol_homol | F58A4:RNAi
|
| WB_Uniquely_mapped |
|
Remark
| Phenotype comment -- Confirmed with independent dsRNA (F58A4.4 -RNA2; similar phenotype). |
| | DIC phenotype -- Slow between pseudocleavage stage and pronuclear envelope breakdown (8 min or more compared to 4-5 min in wild-type); P1 late (5 min or more between division of AB and P1, compared to 2-3 min in wild-type); lack of rotation in P1. |
| | All embryos dead |
|
Results (2)
|