RNAi: TH:335f3   

TH:335f3	RNAi_reagent	TH:335f3
	Method	RNAi
	WormBaseID	WBRNAi00002694
	Reference	WBPaper00004403
	Experiment	Laboratory	TH
		Author (21)
		Date	16 Nov 2000 00:00:00
	Inhibits	Predicted_gene	F58A4.3	Source_evidence	ePCR	867	WS150 Sequence/Annotation Release
		Gene	F58A4.3	Source_evidence	ePCR	1010	WS150 Sequence/Annotation Release
				Map_status	Best_ePCR	III	9614934	9616512
		Transcript	F58A4.3	Source_evidence	ePCR	1010	WS150 Sequence/Annotation Release
		Potential_predicted_gene	F58A4.6	Source_evidence	ePCR	91	WS150 Sequence/Annotation Release
		Potential_Gene	F58A4.6	Source_evidence	ePCR	91	WS150 Sequence/Annotation Release
				Map_status	Bottom50_percentile_ePCR	III	9614934	9616512
		Potential_Transcript	F58A4.6	Source_evidence	ePCR	91	WS150 Sequence/Annotation Release
		WB_Predicted_gene	F58A4.3	Inferred_automatically	RNAi_primary
			F58A4.6	Inferred_automatically	RNAi_primary
		WB_Gene	F58A4.3	Inferred_automatically	RNAi_primary
			F58A4.6	Inferred_automatically	RNAi_primary
		Gene_target	F58A4.3	RNAiDB	ePCR	ePCR_best
				Wormbase	BLAST	BLAST_wormbase_primary
			F58A4.6	RNAiDB	ePCR
				Wormbase	BLAST	BLAST_wormbase_primary
	WB_Homol	Homol_homol	F58A4:RNAi
	WB_Uniquely_mapped
	Remark	Phenotype comment -- Confirmed with independent dsRNA (K.O., unpublished observations; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other CENP-A like gene (F54C8.2) (see BLASTN results). We note, however, that the phenotypes associated with these two genes are somewhat different, suggesting that the phenotype noted here may truly reflect a requirement for F58A4.3 function.
		DIC phenotype -- Daughter nuclei stay close to the central cortex; usually multiple nuclei (karyomeres) in each daughter blastomere.
		DIC phenotype comment -- We have shown in separate work (P.G., unpublished observations) that this phenotype is associated with lagging chromosomal material that retains daughter nuclei together at the central cortex.
		All embryos dead
	Results	Phenotype	Emb
		Phenotype_summary (7)

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