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RNAi: TH:340d5 
| TH:340d5 | RNAi_reagent | TH:340d5 | |||
|---|---|---|---|---|---|
| Method | RNAi | ||||
| WormBaseID | WBRNAi00002723 | ||||
| Reference | WBPaper00004403 | ||||
| Experiment | Laboratory | TH | |||
| Author (21) | |||||
| Date | 16 Nov 2000 00:00:00 | ||||
| Inhibits (5) | |||||
| WB_Homol | Homol_homol | K01G5:RNAi | |||
| WB_Uniquely_mapped | |||||
| Remark | Phenotype comment -- Confirmed with independent dsRNA (K01G5.7-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for K01G5.7 function. | ||||
| DIC phenotype -- Lack of male pronuclear migration; female pronucleus is usually located initially towards center of embryo or next to male pronucleus; sometimes 2 female pronuclei; lack of female pronuclear migration (where applicable, i.e. in those embryos in which the female pronucleus is located away from the male pronucleus); no bipolar spindle assembly. | |||||
| All embryos dead | |||||
| Results | Phenotype | Emb | |||
| Phenotype_summary | Ste | Result | No | ||
| Reason | None_reported | ||||
| Stp | Result | No | |||
| Reason | None_reported | ||||
| Emb | Result | Yes | |||
| EmbPheno | Result | No | |||
| Reason | None_reported | ||||
| PostembLethal | Result | No | |||
| Reason | None_reported | ||||
| PostembPheno | Result | No | |||
| Reason | None_reported | ||||
| Movies_images | Result | No |
