RNAi: TH:340d5
TH:340d5 | RNAi_reagent | TH:340d5 | |
---|---|---|---|
Method | RNAi | ||
WormBaseID | WBRNAi00002723 | ||
Reference | WBPaper00004403 | ||
Experiment | Laboratory | TH | |
Author (21) | |||
Date | 16 Nov 2000 00:00:00 | ||
Inhibits (5) | |||
WB_Homol | Homol_homol | K01G5:RNAi | |
WB_Uniquely_mapped | |||
Remark | Phenotype comment -- Confirmed with independent dsRNA (K01G5.7-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for K01G5.7 function. | ||
DIC phenotype -- Lack of male pronuclear migration; female pronucleus is usually located initially towards center of embryo or next to male pronucleus; sometimes 2 female pronuclei; lack of female pronuclear migration (where applicable, i.e. in those embryos in which the female pronucleus is located away from the male pronucleus); no bipolar spindle assembly. | |||
All embryos dead | |||
Results (2) |