TH:340f10 | RNAi_reagent | TH:340f10
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| Method | RNAi
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| WormBaseID | WBRNAi00002732
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| Reference | WBPaper00004403
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Experiment
| Laboratory | TH
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| |
Author (21)
|
| | Date | 16 Nov 2000 00:00:00 |
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Inhibits
| Gene |
C30C11.2
| Source_evidence | ePCR | 725 | WS150 Sequence/Annotation Release |
| | | | Map_status | Unique_ePCR | III | 8447850 | 8448574 |
| |
Transcript
| C30C11.2.1
| Source_evidence | ePCR | 725 | WS150 Sequence/Annotation Release |
| | | C30C11.2.2
| Source_evidence | ePCR | 725 | WS150 Sequence/Annotation Release |
| | WB_Predicted_gene | C30C11.2
| Inferred_automatically | RNAi_primary |
| | WB_Gene | C30C11.2
| Inferred_automatically | RNAi_primary |
| |
Gene_target
|
C30C11.2
| RNAiDB | ePCR | ePCR_unique |
| | | | Wormbase | BLAST | BLAST_wormbase_primary |
| | | C30C11.2.1
| RNAiDB | ePCR |
| | | C30C11.2.2
| RNAiDB | ePCR |
| WB_Homol | Homol_homol | C30C11:RNAi
|
| WB_Uniquely_mapped |
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Remark
| Phenotype comment -- Confirmed with independent dsRNA (C30C11.2-RNA2; similar phenotype). |
| | DIC phenotype -- In most embryos (e.g. movie1), the male and female pronuclei never become visible, and everything seems to be arrested. Some embryos progressed to the one cell stage (e.g. movie2): in these cases, there is a sriking delay during mitosis (over 20 min between breakdown of the pronuclear envelopes and cleavage furrow ingression, compared to the usual approximately 2 min in wild-type); earlier events in the one cell stage may be slow as well. |
| | DIC phenotype comment -- Staining of fixed embryos with DNA dye shows that fertilization took place, and suggests that at least half of the embryos are arrested during meiotic divisions. |
| | All embryos dead |
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Results
| Phenotype | Emb
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Phenotype_summary (7)
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