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RNAi: TH:340h5   

TH:340h5RNAi_reagentTH:340h5
MethodRNAi
WormBaseIDWBRNAi00002746
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author Gonczy P
Echeverri C
Oegema K
Coulson AR
Jones SJ
Copley RR
Duperon J
Oegema J
Brehm M
Cassin E
Hannak E
Kirkham M
Pichler SC
Flohrs K
Go essen A
Leidel S
Alleaume AM
Martin C
Ozlu N
Bork P
Hyman AA
Date16 Nov 2000 00:00:00
Inhibits (6)
WB_HomolHomol_homolK06H7:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction.
DIC phenotype -- Complex phenotype. In most embryos (e.g. movie1), eggshell appears fragile as witnessed by random yolk granule movements movements; pronuclei appear present, but fragility of embryos prevents thorough phenotypic characterization; no progress to the two cell stage; in other embryos (e.g. movie 2), defect in spindle assembly; also, no visible cleavage furrow; often large cytoplast on anterior side
DIC phenotype comment -- See also phenotypic description in Chase et al. (2000).
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)