RNAiDB Tree View

Gene: W02A11.4

W02A11.4	SMap	S_parent	Sequence	W02A11
	Chromosome	I
	Identity (5)
	Gene_info	Gene_class	uba
		Allele	uCE1-1375
		GO_term (5)
		Structured_description	Provisional_description	uba-2 encodes the C. elegans ortholog of Saccharomyces cerevisiae Uba2p and human ubiquitin-like 2 activating enzyme E1B; by homology, UBA-2 is predicted to form, with a regulatory subunit, AOS-1, a heterodimeric enzyme that activates the ubiquitin-like protein SMO-1/SUMO in preparation for its covalent attachment to target proteins to regulate their subcellular localization and/or stability; in C. elegans, UBA-2 is required for embryogenesis and vulval development, as well as for proper Hox gene regulation; uba-2/aos-1(RNAi) animals that survive embryogenesis show ectopic expression of EGL-5 and ectopic anterior sensory ray formation.	Paper_evidence	WBPaper00005337
						WBPaper00006514
						WBPaper00023983
					Curator_confirmed	WBPerson1843
					Date_last_updated	27 Jan 2005 00:00:00
				In addition, uba-2 and aos-1 may also play a role in fate specification of the VD (ventral D) GABAergic motor neurons, as reduction of uba-2 or aos-1 activity results in ectopic expression of a DD (dorsal D)-specific reporter in the VD neurons.
			Concise_description	uba-2 encodes the C. elegans ortholog of Saccharomyces cerevisiae Uba2p and human ubiquitin-like 2 activating enzyme E1B; by homology, UBA-2 is predicted to form, with a regulatory subunit, AOS-1, a heterodimeric enzyme that activates the ubiquitin-like protein SMO-1/SUMO in preparation for its covalent attachment to target proteins to regulate their subcellular localization and/or stability; in C. elegans, UBA-2 is required for embryogenesis and vulval development, as well as for proper Hox gene regulation; uba-2/aos-1(RNAi) animals that survive embryogenesis show ectopic expression of EGL-5 and ectopic anterior sensory ray formation.
	Molecular_info	Corresponding_CDS	W02A11.4
		Corresponding_CDS_history	W02A11.4a:wp103
			W02A11.4b:wp103
		Corresponding_protein	WP:CE34382
		KOG	KOG2013
		Corresponding_PCR_product	smd_W02A11.4
			sjj_W02A11.4
		Corresponding_RNAi_reagent	Cenix:76-c5
			sjj_W02A11.4
			mv_W02A11.4b
			mv_W02A11.4a
	Experimental_info	RNAi_result	JA:W02A11.4
			Simmer:W02A11.4:Screen_A
			Simmer:W02A11.4:Screen_B
			Cenix:76-c5
		WB_RNAi_result (5)
	Reference	WBPaper00005337
		WBPaper00006514
		WBPaper00023983
		WBPaper00026636
	Remark	Sequence connection from [Jones D, Crowe E, Stevens TA, Candido EPM], 02/06/10 krb.
		W02A11.4a/b isoforms are no longer valid	Person_evidence	WBPerson1847
	Method	Gene