RNAi: WBRNAi00038539
WBRNAi00038539 | RNAi_reagent | probe_1:F11C1 | |||
---|---|---|---|---|---|
WormBaseID | WBRNAi00038539 | ||||
Reference | WBPaper00024419 | ||||
Species | Caenorhabditis elegans | ||||
Experiment | Laboratory | MH | |||
Author (3) | |||||
Date | 01 Sep 2004 00:00:00 | ||||
Genotype | sid-1(qt2) | ||||
Inhibits | WB_Predicted_gene | F11C1.6a | Inferred_automatically | RNAi_primary | |
F11C1.6b | Inferred_automatically | RNAi_primary | |||
WB_Gene | F11C1.6 | Inferred_automatically | RNAi_primary | ||
Gene_target (3) | |||||
WB_Homol | Homol_homol | F11C1:RNAi | |||
Remark | For tissue-specific RNAi, the first 0.9 kb of nhr-25 coding sequence was fused in the sense and antisense orientations to the col-10 promoter, which is expressed in epidermal cells during embryonic and larval stages. Both constructs were injected at 50 ng/mkl, and a sur-5::gfp transcriptional fusion construct was used as a coinjection marker. | ||||
Delivery | Delivered_by | Transgene_expression | |||
Results | Phenotype | Egl | Remark | n=143 | |
Penetrance | 22 | ||||
Mlt | Remark | n=191 | |||
Penetrance | 10 | ||||
Morphology defect | Remark | Animals expressing the pcol-10::nhr-25 sense and antisense constructs in sid-1(qt-2) background sometimes had a shorter body length and were more severely defective in the posterior end. | |||
Phenotype_summary (7) |