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RNAi: WBRNAi00038539   

WBRNAi00038539RNAi_reagentprobe_1:F11C1
WormBaseIDWBRNAi00038539
ReferenceWBPaper00024419
SpeciesCaenorhabditis elegans
Experiment LaboratoryMH
Author (3)
Date01 Sep 2004 00:00:00
Genotypesid-1(qt2)
Inhibits (3)
WB_HomolHomol_homolF11C1:RNAi
RemarkFor tissue-specific RNAi, the first 0.9 kb of nhr-25 coding sequence was fused in the sense and antisense orientations to the col-10 promoter, which is expressed in epidermal cells during embryonic and larval stages. Both constructs were injected at 50 ng/mkl, and a sur-5::gfp transcriptional fusion construct was used as a coinjection marker.
DeliveryDelivered_byTransgene_expression
Results Phenotype Egl Remarkn=143
Penetrance22
Mlt Remarkn=191
Penetrance10
Morphology defect RemarkAnimals expressing the pcol-10::nhr-25 sense and antisense constructs in sid-1(qt-2) background sometimes had a shorter body length and were more severely defective in the posterior end.
Phenotype_summary (7)