RNAi: WBRNAi00038539
WBRNAi00038539 | RNAi_reagent | probe_1:F11C1 | ||||
---|---|---|---|---|---|---|
WormBaseID | WBRNAi00038539 | |||||
Reference | WBPaper00024419 | |||||
Species | Caenorhabditis elegans | |||||
Experiment (4) | ||||||
Inhibits | WB_Predicted_gene | F11C1.6a | Inferred_automatically | RNAi_primary | ||
F11C1.6b | Inferred_automatically | RNAi_primary | ||||
WB_Gene | F11C1.6 | Inferred_automatically | RNAi_primary | |||
Gene_target | F11C1.6a | Wormbase | BLAST | BLAST_wormbase_primary | ||
F11C1.6b | Wormbase | BLAST | BLAST_wormbase_primary | |||
F11C1.6 | Wormbase | BLAST | BLAST_wormbase_primary | |||
WB_Homol | Homol_homol | F11C1:RNAi | ||||
Remark | For tissue-specific RNAi, the first 0.9 kb of nhr-25 coding sequence was fused in the sense and antisense orientations to the col-10 promoter, which is expressed in epidermal cells during embryonic and larval stages. Both constructs were injected at 50 ng/mkl, and a sur-5::gfp transcriptional fusion construct was used as a coinjection marker. | |||||
Delivery | Delivered_by | Transgene_expression | ||||
Results | Phenotype | Egl | Remark | n=143 | ||
Penetrance | 22 | |||||
Mlt | Remark | n=191 | ||||
Penetrance | 10 | |||||
Morphology defect | Remark | Animals expressing the pcol-10::nhr-25 sense and antisense constructs in sid-1(qt-2) background sometimes had a shorter body length and were more severely defective in the posterior end. | ||||
Phenotype_summary | Ste | Result | No | |||
Reason | None_reported | |||||
Stp | Result | No | ||||
Reason | None_reported | |||||
Emb | Result | No | ||||
Reason | None_reported | |||||
EmbPheno | Result | No | ||||
Reason | None_reported | |||||
PostembLethal | Result | No | ||||
Reason | None_reported | |||||
PostembPheno | Result | Yes | ||||
Movies_images | Result | No |