ZK381.4 | SMap | S_parent | Sequence | ZK381
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| Chromosome | IV |
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Identity (5)
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Gene_info
| Gene_class | pgl
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Allele (5)
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Phenotype
| [C.elegansII] ct131 : germline P granules unstained by some(not all) monoclonal antibodies. 5% sterile, Mes if grown at 26C. Cloned: cosmid rescue (B0318). [SS] |
| | | P granules are not stained by K76 or OIC1D4, two anti-P-granule monoclonal antibodies. Mutants display sterility that is temperature-sensitive and has both a maternal and a nonmaternal component. |
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Strain (9)
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GO_term (7)
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Structured_description
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Provisional_description
| pgl-1 encodes a predicted RNA-binding protein that contains a number of C-terminal RGG box motifs; PGL-1 activity is required at high temperatures during midlarval to adult stages for postembryonic germline development and hence fertility, and also for proper formation of the germline-specific P granules; PGL-1 protein, supplied maternally and embryonically, is a constitutive P granule component and thus, its localization is cytoplasmic in oocytes and early embryos, while perinuclear in the germline blastomere P4 and proliferating germ cells of larvae and adults; pgl-1 mRNA is expressed exclusively in hermaphrodite and male germlines and is detectable at all stages of development, with highest levels seen in adults; PGL-1 localization to P granules requires wild-type activity of the predicted germline helicase GLH-1. |
Paper_evidence
| WBPaper00003186
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| | | | | | WBPaper00004672
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| | | | | | WBPaper00004940
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| | | | | Person_evidence | WBPerson1843
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| | | | | Curator_confirmed | WBPerson1843
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| | | | | Date_last_updated | 26 Aug 2004 00:00:00 |
| | | | In vitro, PGL-1 protein binds IFE-1, when assayed either by yeast two-hybrids or by precipitation of GST fusions ('GST-pulldown'). |
| | | Concise_description | pgl-1 encodes a predicted RNA-binding protein that contains a number of C-terminal RGG box motifs; PGL-1 activity is required at high temperatures during midlarval to adult stages for postembryonic germline development and hence fertility, and also for proper formation of the germline-specific P granules; PGL-1 protein, supplied maternally and embryonically, is a constitutive P granule component and thus, its localization is cytoplasmic in oocytes and early embryos, while perinuclear in the germline blastomere P4 and proliferating germ cells of larvae and adults; pgl-1 mRNA is expressed exclusively in hermaphrodite and male germlines and is detectable at all stages of development, with highest levels seen in adults; PGL-1 localization to P granules requires wild-type activity of the predicted germline helicase GLH-1. |
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Molecular_info (5)
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Experimental_info (7)
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Reference (38)
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| Method | Gene
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