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Gene: ZK381.4   

ZK381.4SMapS_parentSequenceZK381
ChromosomeIV
Identity (5)
Gene_info Gene_classpgl
Allele (5)
Phenotype [C.elegansII] ct131 : germline P granules unstained by some(not all) monoclonal antibodies. 5% sterile, Mes if grown at 26C. Cloned: cosmid rescue (B0318). [SS]
P granules are not stained by K76 or OIC1D4, two anti-P-granule monoclonal antibodies. Mutants display sterility that is temperature-sensitive and has both a maternal and a nonmaternal component.
Strain (9)
GO_term (7)
Structured_description Provisional_description pgl-1 encodes a predicted RNA-binding protein that contains a number of C-terminal RGG box motifs; PGL-1 activity is required at high temperatures during midlarval to adult stages for postembryonic germline development and hence fertility, and also for proper formation of the germline-specific P granules; PGL-1 protein, supplied maternally and embryonically, is a constitutive P granule component and thus, its localization is cytoplasmic in oocytes and early embryos, while perinuclear in the germline blastomere P4 and proliferating germ cells of larvae and adults; pgl-1 mRNA is expressed exclusively in hermaphrodite and male germlines and is detectable at all stages of development, with highest levels seen in adults; PGL-1 localization to P granules requires wild-type activity of the predicted germline helicase GLH-1. Paper_evidence WBPaper00003186
WBPaper00004672
WBPaper00004940
Person_evidenceWBPerson1843
Curator_confirmedWBPerson1843
Date_last_updated26 Aug 2004 00:00:00
In vitro, PGL-1 protein binds IFE-1, when assayed either by yeast two-hybrids or by precipitation of GST fusions ('GST-pulldown').
Concise_descriptionpgl-1 encodes a predicted RNA-binding protein that contains a number of C-terminal RGG box motifs; PGL-1 activity is required at high temperatures during midlarval to adult stages for postembryonic germline development and hence fertility, and also for proper formation of the germline-specific P granules; PGL-1 protein, supplied maternally and embryonically, is a constitutive P granule component and thus, its localization is cytoplasmic in oocytes and early embryos, while perinuclear in the germline blastomere P4 and proliferating germ cells of larvae and adults; pgl-1 mRNA is expressed exclusively in hermaphrodite and male germlines and is detectable at all stages of development, with highest levels seen in adults; PGL-1 localization to P granules requires wild-type activity of the predicted germline helicase GLH-1.
Molecular_info (5)
Experimental_info (7)
Reference (38)
MethodGene