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Gene: ZK617.1 
| ZK617.1 | SMap | S_parent | Sequence | SUPERLINK_CB_IV | ||
|---|---|---|---|---|---|---|
| Chromosome | IV | |||||
| Identity (5) | ||||||
| Gene_info | Gene_class | unc | ||||
| Reference_allele | e66 | |||||
| Allele (148) | ||||||
| Phenotype | e66 : twitcher at all stages; (moves slowly with constant trembling); thin; unable to hypercontract and therefore levamisole resistant; Egl; e66/+ twitches in 1% nicotine; ES3 ME0. NA > 200 (s32amber (resembles e66); m52dm; many Tc1 insertions etc.). Most unc-22/+ heterozygotes twitch in nicotine; abnormal muscle structure in most homozygotes; near normal muscle for alleles e105 s12 Tc1 induced alleles. See also unc-54. | |||||
| See also e66, e105, e308, e313, e320, e346, e640, e654, e682, e683, e691, e842, e855, e882, e913, e914, e915, e991, e1090, e1179, e1326, e1923, e2092, e2147, e2148, e2174, e2182, e2377, e2378, e2428, n789, n790, n791, n792, n793, s7, s8, s11, s12, s13, s14, s15, s16, s17, s18, s19, s20, s21, s22, s23, s24, s25, s28, s32, s34, s35, s36, s55, s56, s67, s135, s162, s164, s165, s183, s184, s264, s265, s266, s278, s279, s280, s281, s282, s283, s284, s285, s289, s292, s293, s294, s295, s296, s297, s298, s299, s300, s301, s302, s303, s304, s305, s306, s307, s308, s309, s310, s311, s312, s313, s314, s315, s316, s699, s700, s705, s724, s727, s1787, s1788, s1790, s1791, s1792, s1793, s2028, s2029, s2030, s2038, st136, st137, st192 | ||||||
| [C.elegansII] e66 : twitcher at all stages; (moves slowly with constant trembling); thin; abnormal muscle structure; unable to hypercontract and therefore levamisole resistant; Egl; e66/+ twitches in 1% nicotine; ES3 ME0. OA>200:s32amb (resembles e66), m52dm,e105 (muscle structure near normal),e2428, s7, s12 (near normal muscle structure),s2038,st192 etc. Many Tc1 insertions, plus precise and imprecise excision revertants. Most unc-22/+ heterozygotes twitch in nicotine. Cloned: large gene (>30 kb), transcript ~19 kb,encodes 6049 aa twitchin: single kinase domain, multiple copies of fibronectin-like and Ig-like repeats. Antibody staining indicates twitchin associated with thick filaments. [Waterston et al. 1980; Benian et al. 1989, 1993; GB] | ||||||
| Strain (116) | ||||||
| GO_term (7) | ||||||
| Structured_description | Provisional_description | unc-22 encodes twitchin, a giant intracellular protein with multiple fibronectin- and immunoglobulin-like domains and a single protein kinase domain that is homologous to titin (OMIM:188840); UNC-22 is required in muscle for regulation of the actomyosin contraction-relaxation cycle and for maintenance of normal muscle morphology; UNC-22 associates with myosin and is localized to A-bands; in vitro, UNC-22 can phosphorylate myosin light-chain peptides and can undergo autophosphorylation. | Paper_evidence | WBPaper00001203 | ||
| WBPaper00001773 | ||||||
| WBPaper00005546 | ||||||
| Person_evidence | WBPerson1843 | |||||
| Curator_confirmed | WBPerson1843 | |||||
| Date_last_updated | 17 Jun 2004 00:00:00 | |||||
| Concise_description | unc-22 encodes twitchin, a giant intracellular protein with multiple fibronectin- and immunoglobulin-like domains and a single protein kinase domain that is homologous to titin (OMIM:188840); UNC-22 is required in muscle for regulation of the actomyosin contraction-relaxation cycle and for maintenance of normal muscle morphology; UNC-22 associates with myosin and is localized to A-bands; in vitro, UNC-22 can phosphorylate myosin light-chain peptides and can undergo autophosphorylation. | |||||
| Molecular_info (6) | ||||||
| Experimental_info | RNAi_result | Cenix:155-g4 | ||||
| JA:ZK617.1 | ||||||
| Simmer:ZK617.1 | ||||||
| WB_RNAi_result (5) | ||||||
| Interaction (5) | ||||||
| Reference (290) | ||||||
| Remark | yk16f10.3 removed from sequence list. sdm 11/00 | |||||
| Method | Gene |
