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Gene/ORF B0336.8 (lgg-3) Ace View of B0336.8
B0336.8 Details Help
Description  lgg-3 encodes a hydrophilic protein that is orthologous to Saccharomyces cerevisiae autophagy protein Apg12p; by homology, LGG-3 is predicted to function as a modifier protein required for a protein conjugation system essential for autophagy; in the context of this conjugation system, LGG-3 is predicted to interact with Apg7p and Apg5p homologs (encoded by M7.5 and Y71G12B.12a, respectively) to effect bulk degradation of cytoplasmic components under conditions of starvation or cellular remodeling; free LGG-3 is predicted to localize to the cytoplasm, while LGG-3 conjugated to the Apg5p homolog is predicted to associate with membrane compartments; loss of lgg-3 activity via large-scale RNAi screens results in no obvious developmental or behavioral abnormalities.
Homology Group (KOG)  Protein conjugation factor involved in autophagy
GO Terms  autophagic vacuole formation (GO:0000045) ; cytoplasm (GO:0005737)
Location   III:5697098..5698358
RNAi Phenotypes No phenotypes observed.
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:230-c1
Adult Phenotypes  WT
RNAi Reagent Name & Type Cenix:230-c1 (PCR reagent)
Delivery Method  Injection
Remark  Wildtype results in all three tests.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:310-d5
Adult Phenotypes  WT
RNAi Reagent Name & Type Cenix:310-d5 (PCR reagent)
Delivery Method  Injection
Remark  wild type
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:310-e10
Adult Phenotypes  WT
RNAi Reagent Name & Type Cenix:310-e10 (PCR reagent)
Delivery Method  Injection
Remark  wild type
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:329-c6
Adult Phenotypes  WT
RNAi Reagent Name & Type Cenix:329-c6 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: No significant defects.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:B0336.8
Adult Phenotypes  WT
RNAi Reagent Name & Type sjj_B0336.8 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
TH:310D5
Adult Phenotypes  WTDIC phenotype -- Embryonic development normal
RNAi Reagent Name & Type TH:310D5 (PCR reagent)
Delivery Method  Injection
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
TH:310E10
Adult Phenotypes  WTDIC phenotype -- Embryonic development normal
RNAi Reagent Name & Type TH:310E10 (PCR reagent)
Delivery Method  Injection
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
TH:329C6
Adult Phenotypes  WTDIC phenotype -- Embryonic development normal
RNAi Reagent Name & Type TH:329C6 (PCR reagent)
Delivery Method  Injection
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
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