Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 
Link to C02F5.1RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap
Gene/ORF C02F5.1 (knl-1) Ace View of C02F5.1
C02F5.1 Details Help
Description  knl-1 encodes a novel acidic protein with a coiled-coil region at its C-terminus; KNL-1 is an essential kinetochore component that is required for proper spindle elongation and chromosome separation, and in the kinetochore assembly pathway, plays a key role in linking the initiation of kinetochore formation with the construction of a functional microtubule-binding interface; in the assembly pathway, KNL-1 functions downstream of the DNA-proximal kinetochore components CeCENP-A/HCP-3 and CeCENP-B/HCP-4 and upstream of the outer kinetochore components HIM-10/Nuf2p, NDC-80/HEC1, CeBUB-1, HCP-1, and CeCLASP2/CLS-2; in expression studies in the one-cell embryo, KNL-1 localizes to kinetochores throughout mitosis.
Location   III:8250151..8254258
RNAi Phenotypes
Early_embryo Sister Chromatid Separation abnormal (Cross-eyed) ; Chromosome Segregation (karyomeres) abnormal ; Multiple nuclei
Adult Pvl ; Pvl ; Ste
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:307-c1
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Sister Chromatid Separation abnormal (Cross-eyed)
RNAi Reagent Name & Type Cenix:307-c1 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Sister Chromatid Separation (Cross-eyed)
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:312-f4
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Chromosome Segregation (karyomeres) abnormal
RNAi Reagent Name & Type Cenix:312-f4 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Chromosome Segregation (karyomeres)
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:C02F5.1
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Pvl
RNAi Reagent Name & Type sjj_C02F5.1 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
Simmer:C02F5.1
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_C02F5.1 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
TH:307C1
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:307C1 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (C02F5.1-RNA2; similar DIC phenotype, progeny test not conducted).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Gene Plot of C02F5.1 Browse using GBrowse
Gene Map